Parallel DNA Polymerase Chain Reaction (PD-PCR)

Abstract:

This protocol describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used. Here in FAIRDOM we use this SOP as an example/template

Citation: Protocol Exchange

Date Published: 5th May 2016

Registered Mode: Not specified

Authors: vikash bhardwaj, Vikash bhardwaj, Kulbhushan Sharma

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Citation
bhardwaj, vikash, bhardwaj, V., & Sharma, K. (2016). Parallel DNA Polymerase Chain Reaction (PD-PCR). In Protocol Exchange. Springer Science and Business Media LLC. https://doi.org/10.1038/protex.2016.032
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Created: 26th Nov 2019 at 11:15

Last updated: 28th Jul 2020 at 18:24

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