A transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum–cellular behavior in adaptation to n-butanol

Abstract:

To gain more insight into the butanol stress response of Clostridium acetobutylicum the transcriptional response of a steady state acidogenic culture to different levels of n-butanol (0.25-1%) was investigated. No effect was observed on the fermentation pattern and expression of typical solvent genes (aad, ctfA/B, adc, bdhA/B, ptb, buk). Elevated levels of butanol mainly affected class I heat-shock genes (hrcA, grpE, dnaK, dnaJ, groES, groEL, hsp90), which were upregulated in a dose- and time-dependent manner, and genes encoding proteins involved in the membrane composition (fab and fad or glycerophospholipid related genes) and various ABC-transporters of unknown specificity. Interestingly, fab and fad genes were embedded in a large, entirely repressed cluster (CAC1988-CAC2019), which inter alia encoded an iron-specific ABC-transporter and molybdenum-cofactor synthesis proteins. Of the glycerophospholipid metabolism, the glycerol-3-phosphate dehydrogenase (glpA) gene was highly upregulated, whereas a glycerophosphodiester ABC-transporter (ugpAEBC) and a phosphodiesterase (ugpC) were repressed. On the megaplasmid, only a few genes showed differential expression, e.g. a rare lipoprotein (CAP0058, repressed) and a membrane protein (CAP0102, upregulated) gene. Observed transcriptional responses suggest that C. acetobutylicum reacts to butanol stress by induction of the general stress response and changing its cell envelope and transporter composition, but leaving the central catabolism unaffected.


SEEK ID: https://fairdomhub.org/publications/146

DOI: 10.1016/j.jbiotec.2012.03.018

Projects: COSMIC

Publication type: Not specified

Journal: Journal of Biotechnology

Citation:

Date Published: 1st Mar 2012

Registered Mode: Not specified

Authors: , , Christina Grimmler, ,

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Schwarz, K. M., Kuit, W., Grimmler, C., Ehrenreich, A., & Kengen, S. W. M. (2012). A transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum – Cellular behavior in adaptation to n-butanol. Journal of Biotechnology, 161(3), 366–377. https://doi.org/10.1016/j.jbiotec.2012.03.018
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Created: 4th Apr 2012 at 15:17

Last updated: 4th Apr 2012 at 15:20

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