Assays

What is an Assay?
30 Assays matching the given criteria: (Clear all filters)
Assay class: Experimental assay30

Information about the BioProject submission of all whole-genome-sequenced samples used in this study

Sequencing of 150 bp paired-end reads on an Illumina NextSeq 500 or 2000

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Harvest optical densites and methods of transcriptom, proteom and metabolom samples for all tested substrat conditions.

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Protein copy number at 6h, 12h, 24h, 48h, 72h, 96h, average values and SD for the measurements

Submitter: Niels Zondervan

Assay type: Proteomics

Technology type: Technology Type

Investigation: Modelling of M. pneumoniae metabolism

Study: Proteomics analysis

Metabolomics time series measurements for internal metabolites for 6h, 24h and 48h for multiple experiments. Largely based on MAss spectrometry, bioluminescence kits to measure NAD, NADH at 24h, other time points are infered from relative measurements times the absolute measurements at 24h.

Measurements of external metabolites based on growth curve data. Flux estimates for uptake of external metabolites such as glucose and production rates for external metabolites lactate and acetate

Simple overview of all samples used for training, internal validation by copasi en external validation. Overview of samples metadata, mean metabolite concentration and enzyme concentrations used in the model. Only metabolites present in the model are shown.

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Submitter: Dorothee Houry

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: Manuscript: structural determinants of substrat...

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Submitter: Dorothee Houry

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: Manuscript: structural determinants of substrat...

No description specified

Submitter: Dorothee Houry

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: Manuscript: structural determinants of substrat...

No description specified

Submitter: Dorothee Houry

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: Manuscript: structural determinants of substrat...

No description specified

Submitter: Dorothee Houry

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: 1 hidden item

Study: Manuscript: structural determinants of substrat...

Comparison of Kcat values from the model and values from literature.

Submitter: Niels Zondervan

Assay type: Enzymatic Assay

Technology type: Technology Type

Investigation: Modelling of M. pneumoniae metabolism

Study: Core Model training

No description specified
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