Growth of Col-0 and 35S:miR156 plants in 12hL:12hD
Data for Figures 5D-5F and Supplementary Figure 7B, 7C, including biomass and leaf areas. Image data for leaf areas are included in a .ZIP archive, with two samples as published in 5D. The 'Summary' sheets in the XLSX files include published graphs. Simulation data are included from FMv1. These data were acquired in April 2014, in a separate experiment from the La(er) and Fei-0. Experimental conditions: ∼20.7 °C constant temperature; 12h:12h light/dark cycle; light intensity = 100μmol·m−2·s−1; average daytime CO2 concentration = 405 ppm. Data from 5 plants, so error bars in publication show the SEs of five plants. Biomass and image/area data collected from plants at 37 days after sowing.
SEEK ID: https://fairdomhub.org/assays/711
Experimental assay
Projects: Millar group
Investigation: Arabidopsis Framework Model v1, predicting rosette growth with a multi-model ensemble
Study: Test of FMv1, growth study of other accessions and transgenic line in 12L:12D
Assay position:
Assay type: Cultivation Experiment
Technology type: Cultivation experiment
Organisms: Arabidopsis thaliana : Col-0 wild type (wild-type / wild-type), Arabidopsis thaliana : Pro35S:MIR156 transgenic Arabidopsis (wild-type / wild-type)
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Created: 22nd Aug 2018 at 10:43
Last updated: 22nd Aug 2018 at 13:44
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Projects: Millar group, TiMet
Institutions: University of Edinburgh
https://orcid.org/0000-0002-9258-583X
Projects: Millar group, TiMet, PHYTOCAL: Phytochrome Control of Resource Allocation and Growth in Arabidopsis and in Brassicaceae crops, POP - the Parameter Optimisation Problem, Regulation of flowering time in natural conditions, PlaSMo model repository
Institutions: University of Edinburgh
https://orcid.org/0000-0003-1756-3654
SynthSys is the University of Edinburgh's research organisation in interdisciplinary, Synthetic and Systems Biology, founded in 2012 as the successor to the Centre for Systems Biology at Edinburgh (CSBE).
Projects: Millar group, PHYTOCAL: Phytochrome Control of Resource Allocation and Growth in Arabidopsis and in Brassicaceae crops, TiMet, POP - the Parameter Optimisation Problem, Regulation of flowering time in natural conditions, PlaSMo model repository
Web page: http://www.synthsys.ed.ac.uk
Andrew Millar's research group, University of Edinburgh
Programme: SynthSys
Public web page: http://www.amillar.org
Data, models and simulations for the Chew et al. 2014 paper (PNAS, https://doi.org/10.1073/pnas.1410238111), using wild-type Arabidopsis ecotype Col-0 in standard 12hL:12hD growth conditions, compared to La(er) or Fei-0 accessions, or to plants overexpressing a micro RNA (miR156).
Submitter: Andrew Millar
Studies: Construction of Framework Model v1, Test of FMv1, growth study of Col-0 accession in 12L:12D, Test of FMv1, growth study of Col-0 accession in 5 photoperiods, Test of FMv1, growth study of other accessions and transgenic line in 12...
Assays: Arabidopsis Framework Model v1, Matlab and Simile version, Gas exchange of Fei-0 and Ler plants in 12hL:12hD, Growth of Col-0 and 35S:miR156 plants in 12hL:12hD, Growth of Col-0 in 12hL:12hD, Growth of Col-0 plants in 5 photoperiods, Growth of Fei-0 and Ler plants in 12hL:12hD
Standard growth conditions and 'wild-type' Arabidopsis accessions other than Col-0 and the 35S:miR156 transgenics, with biomass data for whole rosettes, and in some cases, individual leaf area and leaf biomass data
Submitter: Andrew Millar
Provider Name: European Arabidopsis Stock Centre (NASC)
Provider's strain ID: NASC ID: N9952
Organism: Arabidopsis thaliana
Genotypes: wild-type
Phenotypes: wild-type
Comment: From NASC website: Transgenic plants generated by floral dipping method. The homozygous plants are screened in T3. Donation Date: 2010-07-23 Donated by: Max-Planck-Institute for Developmental Biology, Germany: Detlef Weigel lab
binary data file from "Hobo" environment multi-sensor + data logger, located next to the plants used in the experiment. Usually read in HOBOware software, free from Onsetcomp.com. Useful temperature record. Though light levels can usefully show changes they are not well calibrated.
Fig 5D
Figure 5D
Data for Fig. Supp 7B in Chew et al. PNAS 2014 Leaf number of the growing rosettes, from 5 to 37 days after sowing. Data and also results of three FMv1 model simulations, with default, fitted and linear (i.e. no juvenile-adult transition in phyllochron)
Data for Figs. 5D, 5E and Supp 7C in Chew et al. PNAS 2014 FW - fresh weight DW - dry weight (i.e. after baking out all water) SLA - specific leaf area (area per g mass), indicates leaf thickness Indiv, data for individual leaves rather than rosette
Experiment conducted in early April 2014 Intact rosette is pictured, with plant number and genotype in handwritten labels, and ruler for scale. Then dissected leaves are organised in sequence of age, if necessary with small cuts to let them lie flat.
Areas are then measured in image processing.
