Projects: Not specified
Institutions: Not specified
Expertise: Systems Biology, regulation of gene expression, Signal transduction in Gram-negative bacteria; Synthetic Microbiology; Single cell gene expression; Regulatory networks; biochemistry; histidine ki..., stress responses, gene regulation, phenotypic heterogeneity, bistability, sporulation, translational control of gene expression, Bacillus subtilis, Molecular Biology, Genetics, Microbiology
Tools: Time-lapse fluorescence microscopy Flow cytometry, Molecular biology techniques (RNA/DNA), time lapse microscopy, Fluorecence based reporter gene analyses/single cell analyses, PCR, Single Cell analysis, Model organisms, Genetic analysis, Genetic modification, Molecular Biology, Genetics, Microbiology
Expertise: microscopy, fluorescence protein fusions (transcriptional and translational), localisation studies, protein secretion, functional protein expression, stress responses, gene regulation, phenotypic heterogeneity, Bacillus subtilis, Microarray analysis, Molecular Biology, Genetics
Tools: DNA affinity chromatography, EMSA, Molecular biology techniques (RNA/DNA/Protein), transposon mutagenesis, Flow cytometry, Immunofluorescence, Fluorescence microscopy, Site-directed mutagenesis, Fluorecence based reporter gene analyses/single cell analyses, Microarray analysis, Transcriptomics, Genetic modification
PhD student. Analyzing CcpA affinity to cre boxes (catabolite responsive elements) and response of B. subtilis to membrane protein overproduction stress.
Roles: Project Coordinator
The Veening lab is interested in phenotypic bi-stability in Streptococcus pneumoniae and its importance in virulence of this human pathogen.
Tools: Proteomics (2D-PAGE), qPCR, mutant strain generation, Northern analyses), Fluorecence based reporter gene analyses/single cell analyses, purification, molecular biological techniques (RNA/DNA techniques, enzymatic analyses, Protein chemical methods (protein overproduction, quantitative Western analyses)
I am research assistant in the microbiology department at the Ludwig-Maximilians Universität in Munich (München), working at the chair of Prof. Kirsten Jung. In our SysMO consortium we generate biological data and work in close cooperation with the workgroup of Dr. Andreas Kremling of the Max-Planck-Institut für Dynamik komplexer technischer Systeme in Magdeburg who performs mathematical modeling. The topic of our workpackage deals with "K+ homeostasis in Escherichia coli", wherby the K+ transporters,
Tools: molecular biological techniques (RNA/DNA techniques analyses of transport across cell membranes heterologous expression of transporters, mutant strain generation, Fluorecence based reporter gene analyses/single cell analyses, Biochemistry and protein analysis
biochemistry, molecular biology and physiology of lower eukaryotes,
characterization of cell membrane transporters