D.BSRA-240502ALT-1
samples-publish2024-06-04-D.BSRA.xlsx No longer valid
BSRA-Macrophage
MRA_MBL-FcEngineered-EI.xlsx
https://immport.org/shared/study/SDY2541
ea17dee57c0ec75bd0a7890fe1c1d0e9
MD5
BAC-240502ALT-1; TIS-240502ALT-1; AB-220920ALT-1; AB-220921ALT-1; AB-220920ALT-2; AB-220920ALT-3; AB-220920ALT-4; AB-220920ALT-5; AB-220920ALT-6; AB-220920ALT-7; AB-220920ALT-8; AB-220920ALT-9; AB-220920ALT-10; AB-220920ALT-11; AB-220920ALT-12; AB-220920ALT-13; AB-220920ALT-14; AB-220920ALT-15; AB-220920ALT-16; AB-220920ALT-17; AB-220920ALT-18; AB-220920ALT-19; AB-220920ALT-20; AB-220920ALT-21; AB-220920ALT-22; AB-220920ALT-23; AB-220920ALT-24; AB-220920ALT-25; AB-220920ALT-26; AB-220920ALT-27; AB-220920ALT-28; AB-220920ALT-29; AB-220920ALT-30; AB-220920ALT-31; AB-220920ALT-32; AB-220920ALT-33; AB-220920ALT-34; AB-220920ALT-35; AB-220920ALT-36; AB-220920ALT-37; AB-220920ALT-38; AB-220920ALT-39; AB-220920ALT-40; AB-220920ALT-41; AB-220920ALT-42; AB-220920ALT-43; AB-220920ALT-44; AB-220920ALT-45; AB-220920ALT-46; AB-220920ALT-47; AB-220920ALT-48; AB-220920ALT-49; AB-220920ALT-50; AB-220920ALT-51; AB-220920ALT-52; AB-220921ALT-2
Eddie Irvine
2018-04-15T0:0:0+0000
https://fairdomhub.org/sops/729
Macrophage Restriction Assay
mCherry for constitutive expression, GFP under anhydrotetracycline inducible promoter
GFP, mCherry
H37Rv Mtb Live/dead Reporter
1.0x10^6 bacteria per mL of blood
Human monocyte-derived macrophages (MDMs)
N/A
N/A
N/A
N/A
N/A
Fixed and analyzed 3 days post-infection, with additional 16hr post-anhydrotetracycline addition
Columbus Image Data Storage and Analysis System
Revvity
Anhydrotetracycline added at 200 ng/mL to induce GFP expression; monoclonal antibody at 50μg/mL in R10 media without phenol
Creators and SubmitterViews: 51
Created: 6th Jun 2024 at 15:05
Last updated: 18th Sep 2024 at 17:14
TagsThis item has not yet been tagged.
AttributionsNone
Related items
Projects: Hi-IMPAcTB, MIT SRP, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC)
Institutions: Massachusetts Institute of Technology
Projects: Hi-IMPAcTB
Web page: https://www.niaid.nih.gov/research/immune-mechanisms-protection-mycobacterium-tuberculosis
About
This project houses all the publicly available datasets generated by the Hi-IMPAcTB consortium over the course of its 7+ years.
What is Hi-IMPAcTB?
Hi-IMPAcTB is an ambitious endeavor bringing together a team of international, interdisciplinary researchers to improve our understanding of host-pathogen interactions in the context of tuberculosis (TB). Funded by a multi-center contract from the NIH, the long-term goal of the consortium is to enable principled vaccine design by improving ...
Programme: IMPAcTB
Public web page: https://grantome.com/grant/NIH/75N93019C00071-0-9999-1
Submitter: Dikshant Pradhan
Studies: CD4+ T cells are homeostasis regulators during Mtb reinfection, CD8+ lymphocytes are critical for early control of tuberculosis in macaques, Fc-FcγR interactions shift alveolar macrophage metabolism to promote Myc..., Fc-engineered antibodies leverage neutrophils to drive control of Mycoba..., Humoral correlates of protection against Mycobacterium tuberculosis foll..., Immune cells in bronchoalveolar lavage fluid of Ugandan adults who resis..., Multi-modal data integration using Markov Field graphical networks predi..., Multimodal profiling of lung granulomas in macaques reveals cellular cor..., Mycobacterium tuberculosis-specific antibodies detect progression to act..., Robust IgM responses following intravenous vaccination with Bacille Calm..., Specific CD4+ T cell phenotypes associate with bacterial control in peop...
Assays: All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, Anti-Microbial Assay – Metadata, Antibody Challenge - Metadata, Antibody Titer - Data Linked, Antibody Titer Analysis - Data Linked, Antibody-Dependent Cellular Phagocytosis - Data Linked, Antibody-Dependent Cellular Phagocytosis - Data Linked, Antibody-Dependent Cellular Phagocytosis Analysis - Data Linked, Antibody-Dependent NK Cell Activation - Data Linked, Antibody-Dependent NK Cell Activation Analysis - Data Linked, Antibody-Dependent Neutrophil Phagocytosis - Data Linked, Antibody-Dependent Neutrophil Phagocytosis - Data Linked, Antibody-Dependent Neutrophil Phagocytosis Analysis - Data Linked, Antibody-dependent Cellular Phagocytosis - Data Linked, Antibody-dependent Complement Deposition - Data Linked, Antibody-dependent NK Cell Activation - Data Linked, Antibody-dependent Neutrophil Phagocytosis - Data Linked, Bacterial Challenge - Metadata, Bacterial Extraction - Metadata, Bacterial Extraction - Metadata, Bacterial Survivability Restriction Assay - Data Linked, DNA Extraction - Metadata, Digitally Barcoded Mtb Matrix Analysis - Data Attached, Digitally Barcoded Mtb Matrix Analysis - Data Attached, ELISA - Data Linked, FC Receptor Binding - Data Linked, FC Receptor Binding Analysis - Data Linked, FC Receptor Binding Assay - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry Analysis - Data Attached, Flow Cytometry Analysis - Data Linked, Flow Cytometry Analysis - Data Linked, Flow Cytometry Analysis - Data Linked, Flow Cytometry Processing – Data Attached, Flow Cytometry – Data Linked, Functional Assay – Metadata, Glycosylation Assay - Data Linked, Immunohistochemistry - Data Linked, Immunohistochemistry – Data Linked, Library Creation – Metadata, Library Prep - Metadata, Library Prep - Metadata, Linear Mixed Model - Data Linked, Luminex - Data Linked, Luminex Assay – Metadata, Luminex Data Processing – Data Attached, Model Validation, NHP Necropsy – Metadata, NHP Tissue Collection – Metadata, PET-CT Scan - Data Linked, PET-CT Scan Analysis – Data Linked, PET-CT Scan – Data Linked, PET/CT Scan - Data Linked, PET/CT Scan - Data Linked, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit - Metadata, Patient Visit – Metadata, Patient Visit – Metadata, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Single Cell Clustering Analysis - Data Linked, Single Cell Clustering Analysis – Data Linked, Single Cell Expression Analysis - Data Linked, Single Cell Expression Analysis - Data Linked, Single Cell Expression Analysis - Data Linked, Single Cell Expression Matrix Analysis – Data Linked, Single Cell Sequencing Analysis – Data Linked, Single Cell Sequencing – Data Linked, Single Cell Sequencing – Data Linked, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Extraction - Metadata, Tissue Extraction – Metadata, Titer Assay - Data Linked, Titer Assay - Data Linked, Titer Assay Analysis - Data Linked
Edward B. Irvine, Joshua M. Peters, Richard Lu, Patricia S. Grace, Jaimie Sixsmith, Aaron Wallace, Matthew Schneider, Sally Shin, Wiktor Karpinski, Jeff C. Hsiao, Esther van Woudenbergh, Arturo Casadevall, Bryan D. Bryson, Lisa Cavacini, Galit Alter, Sarah M. Fortune doi: https://doi.org/10.1101/2022.05.01.490220
Novel vaccination and therapeutic strategies are urgently needed to mitigate the tuberculosis (TB) epidemic. While extensive efforts have focused ...
Submitter: Jake Schissel
Investigation: IMPAcTB
Assays: All Metadata, Antibody Challenge - Metadata, Antibody-dependent Cellular Phagocytosis - Data Linked, Antibody-dependent Complement Deposition - Data Linked, Antibody-dependent NK Cell Activation - Data Linked, Antibody-dependent Neutrophil Phagocytosis - Data Linked, Bacterial Challenge - Metadata, Bacterial Survivability Restriction Assay - Data Linked, ELISA - Data Linked, Glycosylation Assay - Data Linked, Library Prep - Metadata, Luminex - Data Linked, Patient Visit - Metadata, Single Cell Clustering Analysis - Data Linked, Single Cell Expression Analysis - Data Linked, Tissue Extraction - Metadata
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Download
UID (String) *, Name (String) , File_PrimaryData (String) , Link_PrimaryData (URI) , Checksum_PrimaryData (String) , Checksum_PrimaryType (String) , Parent (String) , Scientist (String) , SampleCreationDate (String) , Protocol (URI) , Type (String) , LightEmission_Plasmid (String) , LightEmission_Reporter (String) , BacterialStrain (String) , MultiplicityOfInfection (String) , InfectedCells (String) , Cells_Vendor (String) , Cells_Catalog# (String) , NumberInfectedCells (String) , ExcitationWavelength (String) , EmissionWavelength (String) , ReadingIntervals (String) , Analyzer (String) , Analyzer_Vendor (String) , Dilution (String)
Not specified
