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B. subtilis_SysMo2_Chemostat_growthrate-salt_salt_+GB_proteome_relative quantification

B. subtilis_SysMo2_Chemostat_growthrate-salt_salt_+GB_proteome_relative quantification
Version 2

B. subtilis was grown in minimal media in a chemostat at growth rate µ=0.1, with 1.2M NaCl and glycine betaine. Relative quantification for the proteome was done using metabolic labeling.

SEEK ID: https://fairdomhub.org/data_files/859?version=2

Filename: B. subtilis_SysMo2_Chemostat_growhtrate-salt_salt_ GB_vs2.xlsx

Format: Spreadsheet

Size: 118 KB

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Views: 2408 Downloads: 89

Created: 12th Jan 2011 at 10:18

Last updated: 22nd May 2014 at 12:11

Last used: 21st May 2020 at 06:38

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Sandra Maass

Projects: BaCell-SysMO

Institutions: University of Greifswald

Expertise: Microbiology, Molecular Biology, Bacillus subtilis, stress responses, functional protein expression, carbon metabolism

Tools: Molecular Biology, Biochemistry and protein analysis, Proteomics, Proteomics (2D-PAGE), 2-D Gel Electrphoresis, GeLC-MS/MS, metabolic labeling, absolute quantification, AQUA

I'm Post-Doc in the lab of Prof. Becher at the University of Greifswald. I'm working on the relative and absolute protein quantitation using gel-based and mass-spectrometric methods.

BaCell-SysMO

BaCell-SysMO 2
Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.

Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying
...

Programme: SysMO

Public web page: http://www.sysmo.net/index.php?index=53

Organisms: Bacillus subtilis

Multiomics study of Bacillus subtilis under osmotic stress

BaCell-SysMO

All contributors

Sandra Maass

High salinity chemostat cultivation, multiomics sampling (proteome, transcriptome, metabolome, fluxome) and modelling of carbon core metabolism of Bacillus subtilis 168.

Snapshots: No snapshots

Studies: B. subtilis_SysMo2_Chemostat_growthrate-salt, Fluxome analysis of Bacillus subtilis 168 under osmotic stress

Assays: 13C Metabolic Flux Analysis of Bacillus subtilis 168 in continuous high-..., Absolute quantification of proteins by the AQUA-technology, Absolute quantification of proteins using QconCAT technology, Relative quantification of proteins by metabolic labeling, Transcriptome data for chemostat cultivated samples, extracellular metabolites, intracellular metabolites

Created: 12th Apr 2011 at 13:23, Last updated: 22nd Nov 2011 at 20:12

B. subtilis_SysMo2_Chemostat_growthrate-salt

BaCell-SysMO

All contributors

Sandra Maass

B. subtilis was grown in minimal media in a chemostat at different growth rates (µ= max, µ=0.1, µ=0.4) and in the presence of 1.2M NaCl (µ=0.1) with or without glycinebetaine. Transcriptome, proteome and metabolome were investigated.

Person responsible: Sandra Maass

Snapshots: No snapshots

Investigation: Multiomics study of Bacillus subtilis under osm...

Assays: Absolute quantification of proteins by the AQUA-technology, Absolute quantification of proteins using QconCAT technology, Relative quantification of proteins by metabolic labeling, Transcriptome data for chemostat cultivated samples, extracellular metabolites, intracellular metabolites

Created: 12th Jan 2011 at 09:50, Last updated: 22nd Nov 2011 at 20:12