168 Trp+
168 Trp+
Not specified
Not specified
Trp+ derivative of B. subtilis 168 wild type.
wild-type
tryptophan prototroph
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Created: 18th May 2010 at 12:45
Last updated: 10th Sep 2014 at 09:03

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Projects: BaCell-SysMO
Institutions: University of Greifswald
I am PhD student at Prof.Uwe Voelker lab in Department of Functional Genomics. My area of research is microbial functional genomics in particular analysing the whole transcriptome(by microarray and other molecular biolology methods) of B.subtilis under various stress conditions. I use QconCAT strategy for absolute quantification of carbon metabolic enzymes via MRM(multiple reaction monitoring) by LC-MS/MS. I also perofrm experiments for understanding of dynamics of SigmaB network for modelling.
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
BaCell-SysMO 2 Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.
Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying ...
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=53
Organisms: Bacillus subtilis
B. subtilis was grown in SMM media with glucose as carbon source and the samples for RNA were harvested OD578nm- 1.0). The stress conditions that were applied over here are growthat 57°C, 16°C, 1.2M Nacl and 37°C(control). All the samples were analysed for transcriptome as biological triplicates.
Submitter: Praveen kumar Sappa
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Redefining the Complete Transcriptome of Bacill...
Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)
SOPs: No SOPs
Data files: Continuous growth_16°C_SMM_Growth curve, Continuous growth_57°C_SMM_Growth curve, Contious growth_1.2M NaCl_SMM_Transcritome_Nimb..., Contious growth_16°C_SMM_Transcritome_Nimblegen, Contious growth_57°C_SMM_Transcritome_Nimblegen, Contiuous growth_37°C_SMM(control)_Growth curve., The condition-dependent transcriptome of Bacill...
Snapshots: No snapshots
Submitter: Praveen kumar Sappa
Assay type: Transcriptomics
Technology type: Technology Type
Investigation: Redefining the Complete Transcriptome of Bacill...
Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)
SOPs: No SOPs
Data files: The condition-dependent transcriptome of Bacill...
Snapshots: No snapshots
B. subtilis was grown in M9 media with glucose as carbon source and the samples were harvested during exponential phase (OD600nm- 0.4), early stationary phase(OD600nm- 1.3), late stationary phase(OD600nm- 1.0). All the samples were analysed for transcriptome as biological triplicates.
Submitter: Praveen kumar Sappa
Assay type: Transcriptomics
Technology type: Microarray
Investigation: Redefining the Complete Transcriptome of Bacill...
Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)
SOPs: SOP for cultivation of B.Subtilis, Total RNA isolation from B.Subtilis
Data files: M9_shake_flask_exp_trans_stat_transcriptome_nim..., The condition-dependent transcriptome of Bacill...
Snapshots: No snapshots
Submitter: Sandra Maass
Assay type: Protein Expression Profiling
Technology type: Mass Spectrometry
Investigation: Multiomics study of Bacillus subtilis under osm...
Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)
SOPs: 1 hidden item
Data files: B. subtilis_SysMo2_Chemostat_growthrate-salt_sa..., B. subtilis_SysMo2_Chemostat_growthrate-salt_sa...
Snapshots: No snapshots
Submitter: Sandra Maass
Assay type: Proteomics
Technology type: Mass Spectrometry
Investigation: Multiomics study of Bacillus subtilis under osm...
Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)
SOPs: No SOPs
Data files: B. subtilis_SysMo2_Chemostat_growhtrate-salt_ce..., B. subtilis_SysMo2_Chemostat_growhtrate-salt_ce...
Snapshots: No snapshots
Submitter: Praveen kumar Sappa
Assay type: Transcriptomics
Technology type: Custom Array
Investigation: Identification of targets of the essential RNas...
Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)
SOPs: RNA isolation and quality check by Agilent bioa..., SOP for cDNA labelling and Microarray hybridizaton
Data files: Trancriptome data - RNA processing in Bacillus ...
Snapshots: No snapshots