Fatty acid contents in feed using Gas chromatography/FAME analysis
Fatty acids are extracted from samples and converted to fatty acid methyl esters (FAMEs) followed by separation by gas chromatography. This yields fatty acid profiles for each sample as percent of total FAME or milligram FAME per gram of biomass.
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Hei Magny,
Takk!
Et par ting:
- vi trenger å få orden på data... Lurer derfor på om du kan du lage ett dokument som inneholder alle resultat fra GCen? Uten grafer, tolkninger etc. Kun data.
- thomas har gjort klar prøver fra saltvann. om dere vil hente dem før ferien hadde det vært supert. Snakk med Jacob om dette.
- har dere analysert prøvene fra leversnitt? Så ikke dem i det siste excel arket du sendte ut. Hvis ferdige, kan du inkludere dem i et eget excell ark?
- vennligst inkluder jon olav, tina, jacob og thomas harvey (cc'a på denne emailen) på eposter angående GC-data. Jeg er på pappaperm og klarer ikke alltid å holde meg oppdatert på e-kommunikasjonsfronten.
Mvh Simen
-- Fra: "Magny Sissel S. Thomassen" Dato: 14. juni 2016 kl. 13.43.57 CEST Til: Simen Rød Sandve Emne: N-3 fettsyrer, små prøver
Jeg fikk ikke bearbeidet prøvene fra Nina før jeg måtte på tur til Bergen med studentene mine forrige uke. Men nå har jeg kikket og regnet (kjapt-ikke garantert), og jeg synes at resultatet er flott med tanke på hvor små prøvene var. Analysen er grei, hvis det er noe problem med slike små prøver, så går det mest på samplingen, at det man tar ut fra de forskjellige er så likt som overhode mulig. Jeg synes både lever-prøver og tarmprøver ser fornuftige ut. Ta en titt på figurene. Det er tydelig at VO-fisken sliter med å få opp nivået på DHA, mens de andre gruppene ikke er veldig forskjellige, men i tarm kan det se ut som VM beholder noe mer av 18:3 n-3 enn i tilsvarende i lever. Muligens er leveren noe flinkere til å omdanne (eller forbrenne) 18:3 n-3. Jeg skal se mer på dataene når jeg får tid. Nå er det full fart med kurs og eksamen frem til 24.
SEEK ID: https://fairdomhub.org/assays/390
Experimental assay
Projects: GenoSysFat
Investigation: Omega-3 metabolism of salmon in relation to diet and genetics
Study: GSF1: Salmon feed-switch experiment vegetable and fish oil 2015-2016
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 15th Jun 2016 at 12:19
Last updated: 20th Aug 2021 at 10:20
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Projects: GenoSysFat, DigiSal
Institutions: Norwegian University of Life Sciences
https://orcid.org/0000-0003-4882-2188
Projects: DigiSal, GenoSysFat
Institutions: Norwegian University of Life Sciences
https://orcid.org/0000-0001-7828-2309
Projects: GenoSysFat, DigiSal
Institutions: Norwegian University of Life Sciences
https://orcid.org/0000-0003-4989-5311
Systems biology for salmon farming is what I do. I lead the DigiSal project (http://tinyurl.com/digisal), whose full title is "Towards the Digital Salmon: From a reactive to a pre-emptive research strategy in aquaculture". DigiSal is part of Digital Life, the first call dedicated to systems biology by the Research Council of Norway. I'm also one of the lead modellers in GenoSysFat (http://tinyurl.com/genosysfat), working to improve the omega-3 content of salmon farmed on sustainable feeds by ...
Salmon farming in the future must navigate conflicting and shifting demands of sustainability, shifting feed prices, disease, and product quality. The industry needs to develop a flexible, integrated basis of knowledge for rapid response to new challenges. The Digital Salmon will be an ensemble of mathematical descriptions of salmon physiology, combining mathematics, high-dimensional data analysis, computer science and measurement technology with genomics and experimental biology into a concerted ...
Projects: GenoSysFat, DigiSal, SEEK tutorial for DigiSal, DigiSal-BT8121
Web page: http://tinyurl.com/digisal
Salmon farmed on modern feeds contains less of the healthy, long-chain fatty acids (EPA and DHA) than before. Up until the turn of the millennium, farmed salmon were fed fish oil as a replacement for their omega-3 rich natural prey. However, fish oil is now a scarce resource, and more than half of the fat in modern feeds comes from plant oils that are inexpensive, but devoid of long-chain omega-3 fatty acids. How can we increase the omega-3 content of salmon on sustainable feeds?
One option is ...
Programme: The Digital Salmon
Public web page: http://tinyurl.com/genosysfat
Atlantic salmon is a main source of essential ω-3 long-chain polyunsaturated fatty acids (LC-PUFA) in many Western diets, especially eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA). However, farmed salmon meat now contains less healthy ω-3 fatty acids (FA) than before, due to the shift from marine to vegetable lipid feed sources. An important future aquaculture challenge is therefore to maintain a healthy and high EPA/DHA content when farmed salmon is fed a vegetable ...
Submitter: Jon Olav Vik
Studies: GSF1: Salmon feed-switch experiment vegetable and fish oil 2015-2016
Assays: Fatty acid contents in feed using Gas chromatography/FAME analysis, Fatty acid contents in tissues using Gas chromatography/FAME analysis, Feed switch 2015-09 Solbergstranda, pilot proteomics, Feed switch 2015-09, 2016-01 Solbergstranda, gross phenotypes, Feed switch 2015-09, 2016-01 Solbergstranda, gut microbiota composition,..., Lipidomics, Metabolomics, Overview of RNAseq datasets in GenoSysFat, RNA sequencing Feed switch- Liver and Gut
This experiment is designed to pinpoint where in the metabolic network there are differences between salmon of different genetic families and on different diets. Analyses of this material will help inform feeding and breeding strategies.
Salmon will be reared on feeds with contrasting levels of very-long-chain polyunsaturated fatty acids. Then some fish will be crossed over to the other diet while others remain as controls. This perturbation of diet should provoke changes in omega-3 metabolism ...
Submitter: Jon Olav Vik
Investigation: Omega-3 metabolism of salmon in relation to die...
Assays: Fatty acid contents in feed using Gas chromatography/FAME analysis, Fatty acid contents in tissues using Gas chromatography/FAME analysis, Feed switch 2015-09 Solbergstranda, pilot proteomics, Feed switch 2015-09, 2016-01 Solbergstranda, gross phenotypes, Feed switch 2015-09, 2016-01 Solbergstranda, gut microbiota composition,..., Lipidomics, Metabolomics, Overview of RNAseq datasets in GenoSysFat, RNA sequencing Feed switch- Liver and Gut
Results of gas chromatography of fatty acid composition of feeds used in Study "GSF1: Salmon feed-switch experiment vegetable and fish oil 2015-2016", https://fairdomhub.org/studies/144.
Feed samples are identified by their EWOS feed number. Fatty acids are identified by ChEBI ID. Numbers in table are mass percentage of lipid fraction.
Details on recipes, pellet size etc for each feed ID are in https://fairdomhub.org/data_files/1308.
(The above clarifications required some detective work, which ...
Creators: Jon Olav Vik, Magny Sidsel Thomassen, Graceline Tina Kirubakaran
Submitter: Jon Olav Vik
Relationship type: Not specified
