Flux Balance Analysis of multi-compartment metabolic model of growing Arabidopsis thaliana

The multi-compartmental metabolic network of Arabidopsis thaliana was reconstructed and optimized in order to explain growth stoichiometry of the plant both in light and in dark conditions. Balances and turnover of energy (ATP/ADP) and redox (NAD(P)H/NAD(P)) metabolites as well as proton in different compartments were estimated. The model showed that in light conditions, the plastid ATP balance depended on the relationship between fluxes through photorespiration and photosynthesis including both cyclic and non-cyclic electron flow. The ATP balance in plastid depended on the ratio between these processes, and therefore can be either deficient, self-supported or producing a surplus. The excess of redox potential from the photosynthetic light system was translocated to the mitochondrion via the malate/oxaloacetate shuttle. The model showed that the mitochondrion consumed protons under both ‘light’ and ‘dark’ conditions and provided ATP to the cytoplasm. Matching the proton fluxes with proton-dependent translocation of sucrose between tissues from source to sink in light and dark conditions corresponded well to the known molecular mechanism of sucrose transport.

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Created: 21st Dec 2015 at 16:05

Last updated: 8th Nov 2017 at 14:21

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