Preparation and transformation of electrocompetent bacterial cells Version 3
Describes the cultivation-, washing- and electroporation-steps required in order to transform bacterial cells with DNA.
SEEK ID: https://fairdomhub.org/sops/315?version=3
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SOP_electro_competent_cells_1_1.docx.pdf
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Created: 4th Mar 2018 at 18:10
Last updated: 12th Apr 2018 at 12:42
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Version 3 (latest) Created 12th Apr 2018 at 12:42 by Maximilian Bahls
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Projects: EmPowerPutida
Institutions: ETH Zurich
Projects that do not fall under current programmes.
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Web page: Not specified
Exploiting native endowments by re-factoring, re-programming and implementing novel control loops in Pseudomonas putida for bespoke biocatalysis. The EmPowerPutida project aims to engineer the lifestyle of Pseudomonas putida to generate a tailored, re-factored chassis for the production of so far non-accessible biological compounds. Pseudomonas putida is a bacterium with a highly versatile metabolism, including the capability to degrade or produce organic chemicals.
Programme: Independent Projects
Public web page: http://www.empowerputida.eu/
