SULFOSYSbiotech_Proteome analysis Version 1
Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis
SEEK ID: https://fairdomhub.org/sops/211?version=1
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SOP_Proteomics_SEEK.pdf
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Created: 5th Feb 2015 at 12:45
Last updated: 20th Oct 2016 at 06:23
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Version 1 (earliest) Created 5th Feb 2015 at 12:45 by Theresa Kouril
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Projects: SulfoSys, SulfoSys - Biotec, Glucose metabolism in cancer cell lines
Institutions: University Duisburg-Essen, Stellenbosch University
Projects: SulfoSys, SulfoSys - Biotec
Institutions: University of Sheffield
Projects: SulfoSys, SulfoSys - Biotec
Institutions: University of Sheffield
I am the foundation Professor of Systems Biology and Engineering within the Department of Chemical and Process Engineering (CPE), at The University of Sheffield. My research philosophy is centred on a mechanistic systems biology approach to solve biochemical reaction engineered processes. I wish to pursue issues involved in the effective utilisation of biological resources. The approach is specifically targeted at the conjunction of chemical engineering (metabolic engineering and synthetic biology), ...
e:Bio - Innovations Competition Systems Biology
Projects: SulfoSys - Biotec, SBEpo - Systems Biology of Erythropoietin
Web page: http://www.fona.de/en/14276
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
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Programme: e:Bio
Public web page: http://www.sulfosys.com/
Integrated systems biology approach including transcriptome, metabolome, proteome analyses and modelling to elucidate amino acid degradation in S. solfataricus P2.
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Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
Integrated systems biology approach including transcriptome, metabolome, biochemistry, proteome analyses and modelling to elucidate the catabolic pathway for L-fucose in S. solfataricus P2.
Submitter: Theresa Kouril
Studies: Comparison of S. solfataricus grown on l-fucose and d-glucose
Assays: Cell free extract activity measurements: L-fuc/d-glc, Metabolic model of Sulfolobus solfataricus, Proteome analysis: d-fuc / l-glu, RNA sequencing:l-fuc/d-glu, intracellular metabolome analysis: l-fucose vs d-glucose
To investigate amino acid degradation pathways in Sulfolobus solfataricus transcriptome, proteome and metabolome analyses were performed on cells grown on caseinhydrolysate as carbon source. Cells grown with glucose served as reference condition. Metabolic modelling was used to compare the efficiency of different degradation routes.
Submitter: Jacqueline Wolf
Investigation: Amino acid degradation in Sulfolobus solfataric...
Assays: Metabolic modelling of S. solfataricus during growth on casaminoacids, Metabolome analysis: Casaminoacids versus D-Glc, Proteome analysis: Casaminoacids versus D-Glc, RNA sequencing: Casaminoacids vs D-glc
Submitter: Theresa Kouril
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
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Submitter: Theresa Kouril
Assay type: Proteomics
Technology type: Tandem Mass Spectrometry
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Submitter: Jacqueline Wolf
Assay type: Proteomics
Technology type: Tandem Mass Spectrometry
Investigation: Amino acid degradation in Sulfolobus solfataric...
