RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y


RNA processing and degradation is initiated by endonucleolytic cleavage of the target RNAs. In many bacteria, this activity is performed by RNase E which is not present in Bacillus subtilis and other Gram-positive bacteria. Recently, the essential endoribonuclease RNase Y has been discovered in B. subtilis. This RNase is involved in the degradation of bulk mRNA suggesting a major role in RNA metabolism. However, only a few targets of RNase Y have been identified so far. In order to assess the global impact of RNase Y, we compared the transcriptomes in response to the expression level of RNase Y. Our results demonstrate that processing by RNase Y results in accumulation of about 550 mRNAs. Some of these targets were substantially stabilized by RNase Y depletion, resulting in half-lives in the range of an hour. Moreover, about 350 mRNAs were less abundant when RNase Y was depleted among them the mRNAs of the operons required for biofilm formation. Interestingly, overexpression of RNase Y was sufficient to induce biofilm formation. The results presented in this work emphasize the importance of RNase Y as the global acting endoribonuclease for B. subtilis.

SEEK ID: https://fairdomhub.org/publications/162

PubMed ID: 21815947

Projects: BaCell-SysMO

Publication type: Not specified

Journal: Mol. Microbiol.


Date Published: 4th Aug 2011

Registered Mode: Not specified

Authors: Martin Lehnik-Habrink, Marc Schaffer, , Christine Diethmaier, Christina Herzberg,

help Submitter

Views: 4254

Created: 2nd May 2012 at 15:21

help Tags

This item has not yet been tagged.

help Attributions


Powered by
Copyright © 2008 - 2022 The University of Manchester and HITS gGmbH

By continuing to use this site you agree to the use of cookies