Assays
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Proton fluxes ensue a change in the membrane potential to which the potassium uptake responds. The membrane potential changes depend on the extrusion of protons, buffering capacities of the media and experimental parametes.
Submitter: Falko Krause
Biological problem addressed: Model Analysis Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Study: Kinetic properties of Trk
Submitter: Katy Wolstencroft
Biological problem addressed: Model Analysis Type
Investigation: Yeast Glycolytic Oscillations
Mutants with a linear respiratory chain consisting of NADH Dehydrogenase II and one of the terminal oxidases cytochrom bo, cytochrome bd I or cytochrome bd II were growth in chemostats with defined oxygen supply. The amounts of biomass formed and of acetate and formate produced were determined.
Submitter: Katja Bettenbrock
Assay type: Experimental Assay Type
Technology type: Chemostat Measurement
Investigation: Analysis of Escherichia coli with linear electr...
Determination of essential amino acids for Streptococcus pyogenes
Submitter: Araz Zeyniyev
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Amino acid metabolism of four LAB species: Stre...
The task of this assay is to determine the impact of oxygen availability on the concentrations of metabolites from different central metabolic pathways. The focus lies on metabolites connected to glycolysis, tri-carbon-acid-cycle and energy metabolism. All strains have been cultured and analysed according to the SOPs listed below
Submitter: Stefan Stagge
Assay type: Metabolomics
Technology type: Liquid Chromatography Mass Spectrometry
Investigation: Steady state studies for different oxygen avail...
This assay describes the determination of concentrations and ratio of metabolites of adenine nucleotides (NAD and NADH). These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.
Submitter: Stefan Stagge
Assay type: Metabolomics
Technology type: Initial Rate Experiment
Investigation: Steady state studies for different oxygen avail...
This assay describes the determination of concentrations and ratio of metabolites of ubiquinones (oxidised and reduced form). These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.
Submitter: Stefan Stagge
Assay type: Metabolomics
Technology type: HPLC
Investigation: Steady state studies for different oxygen avail...
Analytical methods and computational analyses (regression, fitting) will be employed to find properties of the Trk system under different external conditions.
Submitter: Falko Krause
Biological problem addressed: Model Analysis Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Study: Kinetic properties of Trk
Mutants with linear respiratory chains were grown under SUMO chemostat conditions at different defined aerobiosis levels. The ArcA phoshorylation state as determined.
Submitter: Katja Bettenbrock
Assay type: Experimental Assay Type
Technology type: Gel Electrophoresis
Investigation: Analysis of Escherichia coli with linear electr...
Dynamics of extracellular metabolites (glc, pyr, suc, lac, gly, ac, etoh, fum, mal, cit, including loss of akg, g3p, 2pg, 3pg, r5p, f6p, g6p, 6pg) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, etc) were measured.
Submitter: Maksim Zakhartsev
Assay type: Metabolite Profiling
Technology type: HPLC
Investigation: Kinetic analysis of metabolic system using tran...
Dynamics of intracellular metabolites (pyr, suc, fum, mal, akg, pep, g3p, 2pg, 3pg, cit, r5p, f6p, g6p, 6pg, ATP, ADP, AMP, UTP, GTP, inosine, NAD+, IMP, UDP, NADP+, CTP, AdenyloSuccinate, NADPH, trehalose) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, ...
Submitter: Maksim Zakhartsev
Assay type: Metabolite Profiling
Technology type: Gas Chromatography Mass Spectrometry
Investigation: Kinetic analysis of metabolic system using tran...
Dynamics of macromolecules (total RNA) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, etc) were measured.
Submitter: Maksim Zakhartsev
Assay type: Metabolomics
Technology type: Technology Type
Investigation: Kinetic analysis of metabolic system using tran...