Describing of membrane potential changes in response to proton fluxes.
Proton fluxes ensue a change in the membrane potential to which the potassium uptake responds. The membrane potential changes depend on the extrusion of protons, buffering capacities of the media and experimental parametes.
SEEK ID: https://fairdomhub.org/assays/100
Modelling analysis
Projects: TRANSLUCENT
Investigation: TRK1,2 Transport Systems of Saccharomyces cerevisiae
Study: Kinetic properties of Trk
Assay position:
Biological problem addressed: Model Analysis Type
Organisms: Saccharomyces cerevisiae
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Created: 3rd Jun 2010 at 12:22
Last updated: 8th Nov 2017 at 14:20
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Projects: TRANSLUCENT
Institutions: Humboldt-Universität zu Berlin
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Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae
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Cellular parameters of strains lacking both TRK1 and TRK2 genes.
Submitter: Falko Krause
Studies: Bioinformatic studies, Current-voltage relation, Genetic analysis, Key Cell-Physiological Parameters of TRK1,2 Transport Systems Investiga..., Kinetic properties of Trk, Promotor Anaysis, Proteomic analysis, Transcriptional Profile, Transcriptomic Profiling of 14-3-3 Mutants
Assays: 2D Gel Analysis, Current voltage relation for different external KCl, Current-voltage relation for different internal potassium, Describing of membrane potential changes in response to proton fluxes., Determination of kinetic properties based on MIFE flux data and measurem..., In silico Promotor Anaysis, Insilico Promotor Anaysis, Interaction Database Analysis, Internal pH, Membrane Potential, Modelling of the Trk-current, Potassium Concentration, Protein Concentration, Volume
At external potassium concentrations in the range of 10uM to 1mM Trk is major cellular uptake system for potassium. This system responds to the activiy of the proton pump. Transmembrane fluxes of protons and potassium cations are analysed in a signal-response relationship.
