Determination of kinetic properties based on MIFE flux data and measurements of internal pH changes.
Analytical methods and computational analyses (regression, fitting) will be employed to find properties of the Trk system under different external conditions.
SEEK ID: https://fairdomhub.org/assays/101
Modelling analysis
Projects: TRANSLUCENT
Investigation: TRK1,2 Transport Systems of Saccharomyces cerevisiae
Study: Kinetic properties of Trk
Assay position:
Biological problem addressed: Model Analysis Type
Organisms: Saccharomyces cerevisiae
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Created: 3rd Jun 2010 at 12:23
Last updated: 8th Nov 2017 at 14:20
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Projects: TRANSLUCENT
Institutions: Humboldt-Universität zu Berlin
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SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
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Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae
Programme: SysMO
Public web page: http://www.translucent-network.org/
Cellular parameters of strains lacking both TRK1 and TRK2 genes.
Submitter: Falko Krause
Studies: Bioinformatic studies, Current-voltage relation, Genetic analysis, Key Cell-Physiological Parameters of TRK1,2 Transport Systems Investiga..., Kinetic properties of Trk, Promotor Anaysis, Proteomic analysis, Transcriptional Profile, Transcriptomic Profiling of 14-3-3 Mutants
Assays: 2D Gel Analysis, Current voltage relation for different external KCl, Current-voltage relation for different internal potassium, Describing of membrane potential changes in response to proton fluxes., Determination of kinetic properties based on MIFE flux data and measurem..., In silico Promotor Anaysis, Insilico Promotor Anaysis, Interaction Database Analysis, Internal pH, Membrane Potential, Modelling of the Trk-current, Potassium Concentration, Protein Concentration, Volume
At external potassium concentrations in the range of 10uM to 1mM Trk is major cellular uptake system for potassium. This system responds to the activiy of the proton pump. Transmembrane fluxes of protons and potassium cations are analysed in a signal-response relationship.
