To estimate the differnces of internal pH between the wild type, single trk1 and trk2 or double trk1 trk2 mutants, the pH-dependent variant of GFP pHluorin was expressed in cells from a multicopy plasmid, cells were grown under various conditions, and the cell fluorescence was monitored.
SEEK ID: https://fairdomhub.org/assays/81
Investigation: TRK1,2 Transport Systems of Saccharomyces cerevisiae
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
Created: 3rd Jun 2010 at 12:49
Last updated: 8th Nov 2017 at 15:21