Internal pH
To estimate the differnces of internal pH between the wild type, single trk1 and trk2 or double trk1 trk2 mutants, the pH-dependent variant of GFP pHluorin was expressed in cells from a multicopy plasmid, cells were grown under various conditions, and the cell fluorescence was monitored.
SEEK ID: https://fairdomhub.org/assays/81
Experimental assay
Projects: TRANSLUCENT
Investigation: TRK1,2 Transport Systems of Saccharomyces cerevisiae
Study: Key Cell-Physiological Parameters of TRK1,2 Transport Systems Investigation
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Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 3rd Jun 2010 at 10:49
Last updated: 8th Nov 2017 at 14:21
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Projects: TRANSLUCENT
Institutions: Humboldt-Universität zu Berlin
I created this for all SysMo Modellers
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SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae
Programme: SysMO
Public web page: http://www.translucent-network.org/
Cellular parameters of strains lacking both TRK1 and TRK2 genes.
Submitter: Falko Krause
Studies: Bioinformatic studies, Current-voltage relation, Genetic analysis, Key Cell-Physiological Parameters of TRK1,2 Transport Systems Investiga..., Kinetic properties of Trk, Promotor Anaysis, Proteomic analysis, Transcriptional Profile, Transcriptomic Profiling of 14-3-3 Mutants
Assays: 2D Gel Analysis, Current voltage relation for different external KCl, Current-voltage relation for different internal potassium, Describing of membrane potential changes in response to proton fluxes., Determination of kinetic properties based on MIFE flux data and measurem..., In silico Promotor Anaysis, Insilico Promotor Anaysis, Interaction Database Analysis, Internal pH, Membrane Potential, Modelling of the Trk-current, Potassium Concentration, Protein Concentration, Volume
Submitter: Falko Krause
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Assays: Internal pH, Membrane Potential, Potassium Concentration, Protein Concentration, Volume
