HepG2 protein concentration for O2 consumption assay
ExploreProtein measurement used to normalised the oxygen consumption flux measured on an Oroboros oxygraph.
SEEK ID: https://fairdomhub.org/data_files/6350?version=1
Filename:
3, protein_WT_KO_PCoA_Malate_Carnitine_20230307.xlsx
Format: Spreadsheet
Size: 10.6 KB
Export PNG
Creators and SubmitterCreators
Additional credit
Albert Gerding; Nicolette Huijkman; Marcel Vieira-Lara; Anne-Claire Martines
Submitter
Views: 798 Downloads: 34
Created: 7th Mar 2023 at 14:19
Last updated: 13th Jun 2023 at 14:53
TagsThis item has not yet been tagged.
AttributionsNone
Version History
Version 2 (latest) Created 9th Jun 2023 at 11:53 by Christoff Odendaal
No revision comments
Version 1 (earliest) Created 7th Mar 2023 at 14:19 by Christoff Odendaal
No revision comments
Related items
https://orcid.org/0000-0001-6274-3633I am a Professor in Medical Systems Biology and the University Medical Centre Groningen. The research in my lab is focused on complex regulation of mammalian lipid and carbohydrate metabolism, eventually aiming at network-based therapies. We combine dynamic computer simulations with quantitative metabolomics, 13C fluxomics, proteomics and transcriptome analysis, and in depth biochemical analysis. This allows to predict and understand ‘emergent’ properties, those properties that are counterintuitive ...
PoLiMeR is funded through the EU Marie Skłodowska-Curie Innovative Training Network (ITN), which drives scientific excellence and innovation. ITNs bring together universities, research institutes, industry and clinical partners from across the world to train researchers to doctorate level.
Metabolic diseases are a burden on the European population and health care system. It is increasingly recognised that individual differences with respect to history, lifestyle, and genetic make-up affect disease ...
Programme: This Project is not associated with a Programme
Public web page: http://polimer-itn.eu/
Submitter: Christoff Odendaal
Studies: Model analysis, Model construction, Model validation
Assays: ACAD activity partitioning, Comparing acyl-CoA dehydrogenase deficiencies, HepG2 oxygen consumption, Kinetics Minireviews, MCADD patient personalised modelling, MCADD rescue titration, Metabolic control analysis, Models, Predicting urinary acylcarnitines under metabolic decompensation., Whole-body ketogenic flux
Submitter: Christoff Odendaal
Investigation: Mitochondrial fatty acid oxidation in human liver
Assays: ACAD activity partitioning, HepG2 oxygen consumption, Whole-body ketogenic flux
Validation of model's ability to predict oxygen consumption flux as measured usign permeabilised cells in an Oroboros Oxygraph. Generates Fig. 2A in the associated publication.
Download "Model_notebooks.rar", unzip, and run: "2, generate-model-Oroboros-validation-[needs(1)]-20221109.nb" and "4, Fig2A-Oroboros-simulation-data-[needs-(1-2-and-3)]-20221109.nb" after running "1, generate-model-20221109.nb"
Submitter: Christoff Odendaal
Biological problem addressed: Validation
Investigation: Mitochondrial fatty acid oxidation in human liver
Study: Model validation
