Technology type 'Imaging'
Related assays
7 Assays visible to you, out of a total of 19We will compare two different procedures to extract ATP from yeast cells: Standard kit procedure (hot Tris/EDTA) and Serrano procedure (cold perchloric acid). In addition we have tested different condition as it turned out that some are important.
1. Preparation of B. subtilis cultures
Inoculate cells from -80°C stocks in 10 ml time-lapse microscopy (TLM) medium (62 mM K2HPO4 , 44mM KH2PO4, 15 mM (NH4)2SO4, 6.5 mM sodium citrate, 0.8 mM MgSO4, 0.02 % casamino acids, 27.8 mM glucose, 0.1 mM L-tryptophan, the pH was set to 7 using a KOH solution) supplemented with antibiotics, if necessary.
Grow the cells overnight in a shake flask (30°C, 225 rpm).
The following morning, dilute the cells 1:10 in pre-warmed chemically defined medium (CDM) (62
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Submitter: Jan-Willem Veening
Assay type: Experimental Assay Type
Technology type: Imaging
Snapshots: No snapshots
Description of observed phenotypic variables in the MOA investigation.
Submitter: Ziva Ramsak
Assay type: Phenotype observation
Technology type: Imaging
Snapshots: Snapshot 1
Submitter: Ziva Ramsak
Assay type: Phenotype observation
Technology type: Leaf Chamber Fluorometer
Snapshots: No snapshots
tbd
Submitter: Malte Herold
Assay type: Experimental Assay Type
Technology type: Confocal Laser Scanning Microscopy (CLSM)
Snapshots: No snapshots
Submitter: Malte Herold
Assay type: Experimental Assay Type
Technology type: Microscopy
Snapshots: No snapshots