Technology type 'Imaging'

Related assays

7 Assays visible to you, out of a total of 19

We will compare two different procedures to extract ATP from yeast cells: Standard kit procedure (hot Tris/EDTA) and Serrano procedure (cold perchloric acid). In addition we have tested different condition as it turned out that some are important.

No description specified

Submitter: Walter Glaser

Assay type: Metabolomics

Technology type: Imaging

Snapshots: No snapshots

1. Preparation of B. subtilis cultures

Inoculate cells from -80°C stocks in 10 ml time-lapse microscopy (TLM) medium (62 mM K2HPO4 , 44mM KH2PO4, 15 mM (NH4)2SO4, 6.5 mM sodium citrate, 0.8 mM MgSO4, 0.02 % casamino acids, 27.8 mM glucose, 0.1 mM L-tryptophan, the pH was set to 7 using a KOH solution) supplemented with antibiotics, if necessary.
Grow the cells overnight in a shake flask (30°C, 225 rpm).
The following morning, dilute the cells 1:10 in pre-warmed chemically defined medium (CDM) (62

Submitter: Jan-Willem Veening

Assay type: Experimental Assay Type

Technology type: Imaging

Snapshots: No snapshots

Description of observed phenotypic variables in the MOA investigation.

Powered by
Copyright © 2008 - 2020 The University of Manchester and HITS gGmbH