Professor of Microbiology
I'm an 'experimentalist' (molecular microbiologist) Postdoc working on regulation and peptide signaling in Clostridium acetobutylicum.
I'm also a SysMO-DB PAL (Product Application Liason) for COSMIC, working on data management including standards and integration with SysMO SEEK.
Expertise: Systems Biology, regulation of gene expression, Signal transduction in Gram-negative bacteria; Synthetic Microbiology; Single cell gene expression; Regulatory networks; biochemistry; histidine ki..., gene regulation, stress responses, bistability, sporulation, phenotypic heterogeneity, translational control of gene expression, Bacillus subtilis, Molecular Biology, Genetics, Microbiology
Tools: Time-lapse fluorescence microscopy Flow cytometry, time lapse microscopy, Molecular biology techniques (RNA/DNA), Fluorecence based reporter gene analyses/single cell analyses, PCR, Model organisms, Single Cell analysis, Genetic modification, Genetic analysis, Molecular Biology, Genetics, Microbiology
Tools: SubtiWiki, SPINE, bacterial two-hybrid system, Chemical cross-linking, Proteomics (2D-PAGE), mutant strain generation, Genetic analysis, Genetic modification, Biochemistry and protein analysis, Molecular Biology, Microbiology
Tools: protein interaction studies, molecular biological techniques (RNA/DNA techniques), DNA technology RNA technology Protein analysis Fermentation Mutagenesis, gene regulation, Proteomics (2D-PAGE), Model organisms, Proteomics, Transcriptomics, Genetic analysis, Genomics, Cell biology, Biochemistry and protein analysis, Molecular Biology, Microbiology
Optimisation of Bacillus subtilis for the secretion of heterologous proteins Therapeutic proteins (including those required for experimental purposes and clinical trials) are major products of biomanufacturing processes and considerable time and expense are expended to maximise the yield and quality of proteins produced in heterologous hosts. The production host of choice is the Gram-negative bacterium Escherichia coli for which many strains and expression systems have been developed. However,