Expertise: protein secretion, C- and N- metabolism, pathogens, gene regulation, virulence factors, stress responses, metal ion homeostasis, Gram positive bacteria (Bacillus, competence, antimicrobial peptides, Lactococcus, sporulation, phenotypic heterogeneity, Streptococcus), germination, bistability
Group leader Molecular Genetics
Expertise: Systems Biology, regulation of gene expression, Signal transduction in Gram-negative bacteria; Synthetic Microbiology; Single cell gene expression; Regulatory networks; biochemistry; histidine ki..., stress responses, gene regulation, phenotypic heterogeneity, bistability, sporulation, translational control of gene expression, Bacillus subtilis, Molecular Biology, Genetics, Microbiology
Tools: Time-lapse fluorescence microscopy Flow cytometry, Molecular biology techniques (RNA/DNA), time lapse microscopy, Fluorecence based reporter gene analyses/single cell analyses, PCR, Single Cell analysis, Model organisms, Genetic analysis, Genetic modification, Molecular Biology, Genetics, Microbiology
The main area of my expertise concerns protein sorting and secretion in Gram-positive bacteria, such as Bacillus subtilis and Staphylococcus aureus.
The Gram-positive bacterium B. subtilis is well known for its high capacity to secrete proteins into the extracellular milieu, which has led to its exploitation as a "cell factory" for secreted proteins. Nevertheless, the secretion of heterologous proteins of pharmaceutical importance is frequently inefficient. This applied problem has been a major