P.ENG-260831-V1_GammaH2AXStaining.docx Version 1
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P.ENG-260831-V1_GammaH2AXStaining.docx
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Created: 9th Sep 2026 at 16:38
Last updated: 9th Sep 2026 at 16:38
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Version 1 (earliest) Created 9th Sep 2026 at 16:38 by Juanita Pardo Sanchez
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Projects: MIT SRP, Hi-IMPAcTB, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab, NExtSEEK Independent Projects
Web page: https://nextseek.mit.edu/
The MIT Superfund Research Program was created in 2017 to be responsive to communities who live near Superfund Sites. In particular, the MIT team leverages scientific research, community engagement, and educational experiences to support people who are concerned about hazardous chemicals in their environment.
Carcinogenic contaminants threaten the health of people living in the Mystic River Watershed (near the Olin, Industriplex, and Wells G&H Superfund sites) and members of Native American ...
Programme: NExtSEEK
Public web page: Not specified
Submitter: Dikshant Pradhan
Studies: Benthic fluxes of fluorescent dissolved organic material, salt and heat ..., Colorimetric Detection of Aqueous N-Nitrosodimethylamine via Photonitros..., CometChip Enables Parallel Analysis of Multiple DNA Repair Activities, DNA adduct and mutational profiles reveal the saturation point of cellul..., Early life exposure to N-nitrosamine drives genotoxicity, mutagenesis, a..., Electrochemical formation of N-nitrosodimethylamine in nitrite-impacted ..., Excision of mutagenic replication-blocking lesions suppresses cancer but..., Interaction of N-Nitroamines with Bincuelar Copper Complexs for Luminsec..., Low-level N-nitrosamine occurrence in disinfected drinking water and rel..., Molecular origins of mutational spectra produced by the environmental ca..., Novel In Vivo CometChip Reveals NDMA-Induced DNA Damage and Repair in Mu..., Optical Detection of Interleukin-6 using Liquid Janus Emulsions using Hy..., Partitioning parameters of N-nitrosamines: An intercomparison of determi..., Persistent interferon signaling and clonal expansion mark early events i..., Reversible Electrochemical Sensor for NDMA: Leveraging Molecularly Impri..., Sex Differences Paper (Jenny Kay), Sex and base excision repair as key susceptibility factors for alkylatio...
Assays: Absorption and Emission Spectroscopy - Data Linked, Absorption and Emission Spectroscopy Analysis - Data Linked, Agglutination Assay - Data Linked, All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, Atomic Force Microscopy - Data Linked, Chemical Challenge, Chemical Challenge - Metadata, Chemical Challenge - Metadata, Chemical Simulations - Data Linked, Chemical Simulations - Data Linked, Chemical Synthesis - Metadata, Chemical Synthesis - Metadata, Chemical Synthesis - Metadata, Chemical Synthesis - Metadata, Cleaved caspase-3 staining and analysis, Comet Chip - Data Linked, Comet Chip Analysis - Data Attached, Comet Chip Analysis - Data Attached, Crystallography - Data Linked, DNA Adduct Analysis - Data Linked, DNA Extraction - Metadata, DNA Extraction - Metadata, ELISA - Data Linked, Electrochemical Sensor Testing - Data Linked, Electron Paramagnetic Resonance - Data Linked, Experimental Physiochemical Measurement - Data Linked, Experimental Physiochemical Measurement Analysis - Data Linked, Extraction and Library Creation - Metadata, Field Water Sensor Run, Flow Cytometry, Flow Cytometry - Data Linked, Flow Cytometry Analysis, Flow Cytometry Analysis - Data Linked, Fourier Transform Infrared Spectroscopy - Data Linked, GPT Assay, GPT Assay - Data Attached, GPT Assay - Data Linked, GPT Assay - Data Linked, GPT Assay – Data Attached, Gel Permeation Chromatography - Data Linked, Gene Expression Analysis - Data Linked, Genome Alignment - Data Linked, Genome Alignment - Data Linked, High Resolution Mass Spectra - Data Linked, High Resolution Mass Spectra Analysis - Data Linked, HistoPathology Analysis, Illumina Sequencing - Data Linked, Imaging, Imaging - Data Linked, Imaging - Data Linked, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Library Creation - Metadata, Library Prep - Metadata, Library Prep - Metadata, Mass Spectrometry - Data Linked, Mass Spectrometry - Data Linked, Mass Spectrometry - Data Linked, Mass Spectrometry Analysis - Data Linked, Mass Spectrometry Analysis - Data Linked, Mass Spectrometry Processing – Data Linked, Mass Spectrometry – Data Linked, Mouse Challenge - Metadata, Mouse Necropsy – Metadata, Mutational Spectra Analysis - Data Linked, Mutational Spectra Analysis - Data Linked, Mutational Spectral Analysis - Data Attached, Necropsy - Metadata, Nuclear Magnetic Resonance - Data Linked, Nuclear Magnetic Resonance Analysis - Data Linked, Nuclear Magnetic Resonance Spectroscopy - Data Linked, Nuclear Magnetic Resonance Spectroscopy - Data Linked, Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked, Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked, PCR - Data Linked, Pendant Drop Tensiometry - Data Linked, Phosphoproteomics Analysis - Data Linked, Predictive Modeling - Data Linked, Protein Extraction - Metadata, RNA Extraction - Metadata, RNA Isolation - Metadata, RaDR - Data Linked, RaDR Image Machine Learning Analysis, RaDR Image Machine Learning Analysis – Data Attached, Sample Collection - Metadata, Sensor Creation - Metadata, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Single-crystal X-ray Crystallography - Data Linked, Solid Phase Extraction - Metadata, Spatial Transcriptomics - Data Linked, Spatial Transcriptomics Analysis - Data Linked, Tissue Collection, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Collection - Metadata, Tissue Imaging, Tissue Imaging - Data Linked, Tissue Imaging – Metadata, Tissue Lysis – Metadata, Tissue Sectioning, Tissue Sectioning - Metadata, UV-Vis Spectroscopy - Data Linked, UV-Vis Spectroscopy Analysis - Data Linked, Water Quality Detection - Data Linked, Western Blot - Data Linked, Western Blot - Data Linked, Western Blot Analysis - Data Linked, Western Blot Analysis - Data Linked, X-ray Photoelectron Spectroscopy - Data Linked, γH2AX staining and analysis
Kay et al. 2026, DNA Repair 166 (2026) 103955 (DOI: 10.1016/j.dnarep.2026.103955; PMID: 42566817). A study of how sex and the Aag base-excision-repair gene modulate NDMA-induced liver damage, mutagenesis and tumourigenesis in mice.
Assays, data files and SOPs will be added as the deposit progresses. Reach out to Jenny Kay (kay@silentspring.org) with any questions.
Submitter: Juanita Pardo Sanchez
Investigation: MIT SRP
Assays: Chemical Challenge, Cleaved caspase-3 staining and analysis, Flow Cytometry, Flow Cytometry Analysis, GPT Assay, HistoPathology Analysis, Imaging, RaDR Image Machine Learning Analysis, Tissue Collection, Tissue Imaging, Tissue Sectioning, γH2AX staining and analysis
Submitter: Juanita Pardo Sanchez
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
