Data files

Steady state concentrations of intracellular metabolites in yeast Saccharomyces cerevisiae anaerobic chemostat at D = 0.1 h-1 on minimal medium. All metabolite concentrations are in mmol/L(CV).

Steady state concentrations of extracellular metabolites in yeast Saccharomyces cerevisiae anaerobic chemostat at D = 0.1 h-1 on minimal medium. All metabolite concentrations are in mmol/L(R) except CO2, which is in parts of the partial pressure.

Steady state metabolic fluxes measured in glucose-limited chemostat of Saccharomyces cerevisiae at D = 0.1 h-1 growing on minimal medium. Fluxes are: glucose, ethanol, glycerol, acetate, succinate, pyruvate, lactate, citrate, malate, a-ketoglutarate, fumarate

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Monitoring of end products ethanol, acetone, butanol, acetate and butyrate during master fermentation of Clostridium acetobutylicum at the steady state pH values 5.5, 5.3, 5.1, 4.9 and 4.7.

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Confocal microscopy of CIN5-GFP from the collection of GFP strains at standard growth conditions (50 mM KCl)

Confocal microscopy of HOG1-GFP from GFP-strain collection under standard growth cionditions (50 mM KCl).

Confocal microscopy of Pma1-GFP cells after 60 min at 0 or 50 mM KCl .

Confocal microscopy of Nha1-GFP cells after 60 min at 0 or 50 mM KCl or at 1 M NaCl.

Confocal microscopy of Arl1-GFP cells after 60 min at 0 or 50 mM KCl or at 1 M NaCl.

Confocal microscopy of Ena1-GFP cells after 60 min at 0 or 50 mM KCl or at 1 M NaCl.

Micro array analysis of steady-state growing cells of Clostridium acetobutylicum at pH 5.7 (acidogenesis) in comparison to pH 4.5 (solventogenesis).

Monitoring of end products in mM of ethanol, acetone, butanol, acetate and butyrate during master fermentation of Clostridium acetobutylicum at the steady state pH values 5.7 (acidogenesis) and 4.5 (solventogenesis).

TRK1, TRK2

Micro array analyses of all mRNA levels of cells growing at steady state pH values 5.7, 5.5, 5.3, 5.1, 4.9, 4.7 compared to the same reference steady-state pH 4.5.

All spots and identified proteins of Clostridium acetobutylicum growing at steady-state pH 5.7 (acidogenesis) and pH 4.5 (solventogenesis), respectively, using 2D PAGE and Maldi-TOF analysis. The focus were cytosolic proteins with an isoelectric point bewteen 4 and 7 as well as a molecular weight of 180-10 kDa.

Representation of all prepared 2D PAGE analyses of cytosolic proteins of Clostridium acetobutylicum growing continuously at pH 5.5, 5.3, 5.1, 4.9 and 4.7.

Growth yeast with 50mM K+ and short timing collection

Growth yeast with 50mM K+ and long timing collection

Growth yeast with 0mM K+ and short timing collection

Growth yeast with 0mM K+ and short timing collection

Growth yeast with 50mM K+ and short timing collection

Growth yeast with 0mM K+ and short timing collection

Growth yeast with 0mM K+ and long timing collection

Growth yeast with 50mM K+ and short timing collection