Data files

The pdf-file shows simulations of a hypothetical model of sigma factor competition. It simulates the dynamics that we can expect from the experiments and prepares for the analysis of the experimental data. Analysis of sigma factor competition is based on a Lineweaver-Burk representation of RNApolymerase and competing sigma factors.

S. pneumoniae RNA-Seq Barcodes: HPUra 1: CAGATC HPUra 2: ACTTGA Kanamycin: AGTCAA

S. pneumoniae RNA-Seq Barcodes: Control 1: CAGATC Control 2: ACTTGA

E. coli RNA-Seq Barcodes: Trimethoprim 1: CCGTCC Trimethoprim 2: GTAGAG

E. coli RNA-Seq Barcodes: Control 1: AGTTCC Control 2: ATGTCA

E. coli DNA-Seq Barcodes: control 1: GTGGCC control 2: GTTTCG Trimethoprim 1: CACTCA Trimethoprim 2: CAGGCG

S. pneumoniae kanamycin DNA PE2

S. pneumoniae kanamycin DNA PE1

S. pneumoniae HPUra 2 DNA PE2

S. pneumoniae HPUra 2 DNA PE1

S. pneumoniae HPUra 1 DNA PE2

S. pneumoniae HPUra 1 DNA PE1

S. pneumoniae Control 2 DNA PE2

S. pneumoniae Control 2 DNA PE1

S. pneumoniae Control 1 DNA PE2

S. pneumoniae Control1 DNA-Seq PE1

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Meeting location map

Monitoring of partial pressure of CO2 in off-gas. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, etc) were measured.

Dynamics of intracellular metabolites (pyr, suc, fum, mal, akg, pep, g3p, 2pg, 3pg, cit, r5p, f6p, g6p, 6pg, ATP, ADP, AMP, UTP, GTP, inosine, NAD+, IMP, UDP, NADP+, CTP, AdenyloSuccinate, NADPH, trehalose) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, ...

Dynamics of extracellular metabolites (glc, pyr, suc, lac, gly, ac, etoh, fum, mal, cit, including loss of akg, g3p, 2pg, 3pg, r5p, f6p, g6p, 6pg) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, etc) were measured.

Kinetic characterisation of FBPAase. Expermental data for enzyme reaction rates with increasing concentrations of DHAP and GAP.

Kinetic characterisation of TPI. Experimental data for enzyme reaction rates with increasing concentrations of DHAP and GAP, and inhibition with 3PG and PEP.

Kinetic characterisation of GAPDH. Expermental data for enzyme reaction rates with increasing concentrations of BPG, NADPH, NADP, GAP and Pi.

Experimental data for determination of half-life of gluconeogenic intermediates (BPG, GAP and DHAP).

Kinetic characterisation of PGK. Expermental data for enzyme reaction rates with increasing concentrations of ATP, ADP, BPG and 3PG.