Paul Heusden

Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae

Investigation of the role of 14-3-3 proteins in the S. cerevisiae cation homeostasis

Selected strains from the collection of GFP-tagged S. cerevisiae strains are cultivated at different extracellular caion concentrations and the localization of the GFP-tagged protein will be studied by confocal microscopy. The effect of deletion of the BMH1 gene, encoding the major 14-3-3 isoform, will be analyzed.

Confocal microscopy of CIN5-GFP from the collection of GFP strains at standard growth conditions (50 mM KCl)

Confocal microscopy of HOG1-GFP from GFP-strain collection under standard growth cionditions (50 mM KCl).

Confocal microscopy of Pma1-GFP cells after 60 min at 0 or 50 mM KCl .

Confocal microscopy of Nha1-GFP cells after 60 min at 0 or 50 mM KCl or at 1 M NaCl.

Confocal microscopy of Arl1-GFP cells after 60 min at 0 or 50 mM KCl or at 1 M NaCl.

Confocal microscopy of Ena1-GFP cells after 60 min at 0 or 50 mM KCl or at 1 M NaCl.

PMA1

PPZ1

GCN4