Metabolic changes by oxidative stress in T. b. brucei 427 Version 2
Untargeted and targeted metabolic analysis on T. b. brucei 427 grown under oxidative stress with methylene blue has been carried out. This work has been completed with 11 bio-reps and found significant metabolic changes as you can see in the IDEOM file attached. 'Comparison' tab in the data spread sheet shows heat maps and fold change analysis regarding different metabolite levels (T: T brucei, TMB: T. brucei exposed to methylene blue, numbers: time points, 0, 5, 60 & 120min). If you double click any metabolite name (column E), then you will be able to see the graph showing the metabolite level. If you want to understand more about IDEOM, please see the below reference as IDEOM has been recently published in Bioinformatics. Through multivariate and univariate analysis, a potential oxidative stress maker has been found since the level of ariginine phosphate dramatically decreased as methylene blue concentration increased. The participation of the phosphagen system related to arginine kinase in oxidative stress responses has been already reported (C. A. Pereira et al., 2003, FEBS Letters, 554, 201-205). Arginine kinase kock-out (ArgK KO) cells have been already produced and it is under investigation if there are any different drug responses between ArgK KO and wild type cells.
For IDEOM: D. J. Creek et al., 2012, IDEOM: An Excel interface for analysis of LC-MS based metabolomics data, Bioinformatics.
SEEK ID: https://fairdomhub.org/data_files/923?version=2
Filename:
IDEOM_v11_neg_posneg_comparison.xlsb
Format: Excel 2007 binary spreadsheet
Size: 26.9 MB
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Created: 26th Apr 2012 at 14:22
Last updated: 11th Jan 2013 at 16:15
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Version 2 (latest) Created 26th Apr 2012 at 14:22 by Dong-Hyun Kim
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The aims of this investigation is to quantify metabolites associated with pathways involved in stress responses for parameterising models of oxidative stress metabolism; the measurement of metabolic fluxes of metabolites of interest with intracellular concentrations
Submitter: Dong-Hyun Kim
Studies: Metabolic flux measurement, Targeted metabolite analysis, Untargeted metabolite analysis
Assays: Generation of uniformly 13C-labelled E. coli extract, Intracellular metabolite concentrations in T. brucei exposed to oxidativ..., Intracellular metabolite concentrations in T. brucei under pH stress, LC-MS based absolute quantification of extracellular metabolites, LC-MS based absolute quantification of intracellular metabolites, Metabolite profiling on T. brucei exposed to oxidative stress
In addition to the highly targeted quantification of metabolites already known to play major roles in oxidative stress, to provide data directly compatible with current models, we will also take an untargeted metabolomics approach. This will enable us to identify other areas of the metabolome influenced by, or influencing, oxidative stress and will allow us to compare changes in each of the stress-inducing stimuli. We have recently pioneered untargeted metabolite profiling of T. brucei using ...
Metabolite profiling on T. brucei exposed to methylene blue has been carried out using LC-MS to investigate metabolic changes caused by oxidative stress
Submitter: Dong-Hyun Kim
Assay type: Metabolite Profiling
Technology type: Liquid Chromatography Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
