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Martin Lehnik-Habrink, Marc Schaffer
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Created: 14th Dec 2012 at 17:25
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Version 1 (earliest) Created 14th Dec 2012 at 17:25 by Praveen kumar Sappa
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Projects: BaCell-SysMO
Institutions: University of Greifswald
Projects: BaCell-SysMO
Institutions: University of Greifswald
I am PhD student at Prof.Uwe Voelker lab in Department of Functional Genomics. My area of research is microbial functional genomics in particular analysing the whole transcriptome(by microarray and other molecular biolology methods) of B.subtilis under various stress conditions. I use QconCAT strategy for absolute quantification of carbon metabolic enzymes via MRM(multiple reaction monitoring) by LC-MS/MS. I also perofrm experiments for understanding of dynamics of SigmaB network for modelling.
Projects: BaCell-SysMO
Institutions: University of Goettingen
Expertise: Microbiology, Genetics, Molecular Biology, Bacillus subtilis, regulation of gene expression, protein-protein interactions, Mycoplasma pneumoniae, Cell physiology, protein-RNA interactions
Tools: Microbiology, Molecular Biology, Biochemistry and protein analysis, Genetic analysis, Genetic modification, Proteomics (2D-PAGE), mutant strain generation, Chemical cross-linking, SubtiWiki, SPINE, bacterial two-hybrid system
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
BaCell-SysMO 2 Modelling carbon core metabolism in Bacillus subtilis – Exploring the contribution of protein complexes in core carbon and nitrogen metabolism.
Bacillus subtilis is a prime model organism for systems biology approaches because it is one of the most advanced models for functional genomics. Furthermore, comprehensive information on cell and molecular biology, physiology and genetics is available and the European Bacillus community (BACELL) has a well-established reputation for applying ...
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=53
Organisms: Bacillus subtilis
The aim of the study is to assess the global function of RNase Y in RNA processing and degradation in Bacillus subtilis. To this end we constructed a strain allowing controlled depletion of RNase Y and used microarrays to analyze the transcriptome in response to the expression level of RNase Y.
Submitter: Praveen kumar Sappa
Studies: Identification of targets of the essential RNase Y of Bacillus subtilis
Assays: Transcritome data_Identification of targets of the essential RNase Y of ...
Snapshots: No snapshots
The aim of the study is to assess the global function of RNase Y in RNA processing and degradation in Bacillus subtilis. To this end we constructed a strain allowing controlled depletion of RNase Y and used microarrays to analyze the transcriptome in response to the expression level of RNase Y.
Submitter: Praveen kumar Sappa
Investigation: Identification of targets of the essential RNas...
Assays: Transcritome data_Identification of targets of the essential RNase Y of ...
Snapshots: No snapshots
Submitter: Praveen kumar Sappa
Assay type: Transcriptomics
Technology type: Custom Array
Investigation: Identification of targets of the essential RNas...
Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)
SOPs: RNA isolation and quality check by Agilent bioa..., SOP for cDNA labelling and Microarray hybridizaton
Data files: Trancriptome data - RNA processing in Bacillus ...
Snapshots: No snapshots