Expertise: protein secretion, C- and N- metabolism, gene regulation, stress responses, pathogens, virulence factors, metal ion homeostasis, Gram positive bacteria (Bacillus, Lactococcus, Streptococcus), competence, sporulation, germination, antimicrobial peptides, phenotypic heterogeneity, bistability
Group leader Molecular Genetics
Expertise: microscopy, fluorescence protein fusions (transcriptional and translational), localisation studies, protein secretion, functional protein expression, gene regulation, stress responses, phenotypic heterogeneity, Bacillus subtilis, Microarray analysis, Molecular Biology, Genetics
Tools: DNA affinity chromatography, EMSA, Molecular biology techniques (RNA/DNA/Protein), transposon mutagenesis, Fluorescence microscopy, Flow cytometry, Immunofluorescence, Site-directed mutagenesis, Fluorecence based reporter gene analyses/single cell analyses, Microarray analysis, Transcriptomics, Genetic modification
PhD student. Analyzing CcpA affinity to cre boxes (catabolite responsive elements) and response of B. subtilis to membrane protein overproduction stress.
The main area of my expertise concerns protein sorting and secretion in Gram-positive bacteria, such as Bacillus subtilis and Staphylococcus aureus.
The Gram-positive bacterium B. subtilis is well known for its high capacity to secrete proteins into the extracellular milieu, which has led to its exploitation as a "cell factory" for secreted proteins. Nevertheless, the secretion of heterologous proteins of pharmaceutical importance is frequently inefficient. This applied problem has been a major