Studies

22 Studies visible to you, out of a total of 22

Multi experimental approach to define the gene expression remodelling under potassium starvation conditions.

How do the fluxes of rubidium (potassium) change during potassium starvation.

What is the proteome of starved cells. Main characteristics?

No description specified

Are there one or several stationary states for physiological parameters dependent on external KCl? For which range of external potassium the cell is able to maintain a constant pH, potassium content, membrane potential and volume?

No description specified

Submitter: Falko Krause

Investigation: K+ Starvation in Saccharomyces cerevisiae

Assays: No Assays

No description specified

Submitter: Falko Krause

Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...

Assays: No Assays

No description specified
No description specified

How does the current mediated by Trk1,2 depend on external and internal ion concentrations? How is the membrane potential shifted by the concentrations of various ions, especially ammonium?

Does the general proteome of yeast cells change in a strain lacking Trk1,2 transporter? Under which conditions?

Studies on the effect of deletion of genes encoding 14-3-3 proteins on the transcriptional response of yeast cells to potassium starvation.

Submitter: Falko Krause

Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...

Assays: No Assays

Studies on genetic interactions between genes encoding 14-3-3 proteins and genes encoding transporters to elucidate which transporter(s) are regulated by 14-3-3 proteins.

Submitter: Falko Krause

Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...

Assays: No Assays

Bioinformatic studies to elucidate the role of 14-3-3 proteins in cation homeostasis.

How does the flux of potassium, hydrogen change during potassium uptake? Which ions are involved in maintaining the required charge balance?

How does the transport of potassium and hydrogen ions effect the concentrations in the near surrounding of the cell.

How does the current mediated by Trk1,2 depend on external and internal ion concentrations? How is the membrane potential shifted by the concentrations of various ions, especially ammonium?

At external potassium concentrations in the range of 10uM to 1mM Trk is major cellular uptake system for potassium. This system responds to the activiy of the proton pump. Transmembrane fluxes of protons and potassium cations are analysed in a signal-response relationship.

Selected strains from the collection of GFP-tagged S. cerevisiae strains are cultivated at different extracellular caion concentrations and the localization of the GFP-tagged protein will be studied by confocal microscopy. The effect of deletion of the BMH1 gene, encoding the major 14-3-3 isoform, will be analyzed.

Submitter: Paul Heusden

Investigation: Role of 14-3-3 proteins in Saccharomyces cerevi...

Assays: No Assays

To model the ENA1 transcriptional regulation a model has to be established. First this will be just a graphical representation, it shall then be extended to a boolean model and shall at one point be converted to a kinetic model.

Cells are starved. Potassium and subsequently glucose are added to the medium. Proton and potassium fluxes across the plasma membrane are recorded before and after these events for WT and mutants lacking specific transporter proteins.

Submitter: Simon Borger

Investigation: K+ Starvation in Saccharomyces cerevisiae

Assays: No Assays

Powered by
(v.1.12.2)
Copyright © 2008 - 2022 The University of Manchester and HITS gGmbH

By continuing to use this site you agree to the use of cookies