Time courses of the internal pH changes under the conditions of Navarrete et al. (2010) will be obtained. The usage of different mutants (transport systems and regulation factors) will reveal the influence and key systems of pH regulation.
To estimate the changes of internal pH, the pH-dependent variant of GFP pHluorin is expressed in cells from a multicopy plasmid, and the changes in cell fluorescence are monitored during 5 hours of incubation in YNB-F growth media without added potassium.
SEEK ID: https://fairdomhub.org/assays/61
Investigation: K+ Starvation in Saccharomyces cerevisiae
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
Created: 3rd Jun 2010 at 12:18
Last updated: 8th Nov 2017 at 15:21