Time courses of the internal pH changes
Time courses of the internal pH changes under the conditions of Navarrete et al. (2010) will be obtained. The usage of different mutants (transport systems and regulation factors) will reveal the influence and key systems of pH regulation.
To estimate the changes of internal pH, the pH-dependent variant of GFP pHluorin is expressed in cells from a multicopy plasmid, and the changes in cell fluorescence are monitored during 5 hours of incubation in YNB-F growth media without added potassium.
SEEK ID: https://fairdomhub.org/assays/61
Experimental assay
Projects: TRANSLUCENT
Investigation: K+ Starvation in Saccharomyces cerevisiae
Study: Key Cell-Physiological Parameters
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Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 3rd Jun 2010 at 10:18
Last updated: 8th Nov 2017 at 14:21
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Projects: TRANSLUCENT
Institutions: Humboldt-Universität zu Berlin
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SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae
Programme: SysMO
Public web page: http://www.translucent-network.org/
Experimental approaches to evaluate cellular response to Potassium limitation. For this purpose, a series of isogenic strains derived from BY4741 and lacking one (TRK1 or TRK2) or both TRK genes encoding specific potassium uptake systems was constructed using the homologous recombination and Cre-lox system.
Submitter: Simon Borger
Studies: Analysis of proton and potassium fluxes, Experimental approaches to determine the dependence of volume, pH and me..., Ion Flux Changes, Key Cell-Physiological Parameters, Long Term Metabolomic Profile, Proteomic Studies, Pysiological Model, Transcriptional Profile
Assays: 2D-Gel Electrophoresis, Ammonium fluxes, External Potassium Concentration, External pH changes, HPLC-MS, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, LacZ Reporters, Membrane Potential, NMR, Potassium fluxes, Protein Concentration, Protein Identification - MS, Proton fluxes, RT-PCR, Stable membrane potential, Stable pH, Stable potassium concentration, Stable volume, Time Course Micro Array Experiment, Time courses of the internal pH changes, Volume determination during starvation at different times.
How does the Volume, internal pH, membrane potential and intracellular cation content change in a time dependent scale during potassium limitation.
Submitter: Falko Krause
Investigation: K+ Starvation in Saccharomyces cerevisiae
Assays: External Potassium Concentration, External pH changes, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, Membrane Potential, Protein Concentration, Time courses of the internal pH changes, Volume determination during starvation at different times.
