Volume determination during starvation at different times.
Is cell volume affected by potassium starvation?
The volume reduction as a response to the shift of cells from a medium of high potassium to potassium free medium will be studied in mutants lacking certain membrane transporters like Trk1,2 Nha1, etc. The conditions for the experiments follow Navarrete et al. (2010). Additionally knockouts of related regulation proteins (SAP155, SAP185) will be tested. For each mutant several time points will be measured to generate time courses.
SEEK ID: https://fairdomhub.org/assays/60
Experimental assay
Projects: TRANSLUCENT
Investigation: K+ Starvation in Saccharomyces cerevisiae
Study: Key Cell-Physiological Parameters
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
Export PNG
Download
Creators and Submitter
Views: 2376
Created: 3rd Jun 2010 at 10:17
Last updated: 8th Nov 2017 at 14:21
TagsThis item has not yet been tagged.
Related items
Projects: TRANSLUCENT
Institutions: Humboldt-Universität zu Berlin
I created this for all SysMo Modellers
http://www.semanticsbml.org/aym Annotate Your Model
There you can annotate your non SBML models with biological terms (MIRIAM annotations). As a cool extra you can view you model source code with inserted biological infomation.
Together with this http://www.semanticsbml.org/semanticSBML you can serach for similar BioModels. The similarity search is based on MIRIAM annotations that are attached to you model. AYM also allows you to create annotations without ...
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae
Programme: SysMO
Public web page: http://www.translucent-network.org/
Experimental approaches to evaluate cellular response to Potassium limitation. For this purpose, a series of isogenic strains derived from BY4741 and lacking one (TRK1 or TRK2) or both TRK genes encoding specific potassium uptake systems was constructed using the homologous recombination and Cre-lox system.
Submitter: Simon Borger
Studies: Analysis of proton and potassium fluxes, Experimental approaches to determine the dependence of volume, pH and me..., Ion Flux Changes, Key Cell-Physiological Parameters, Long Term Metabolomic Profile, Proteomic Studies, Pysiological Model, Transcriptional Profile
Assays: 2D-Gel Electrophoresis, Ammonium fluxes, External Potassium Concentration, External pH changes, HPLC-MS, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, LacZ Reporters, Membrane Potential, NMR, Potassium fluxes, Protein Concentration, Protein Identification - MS, Proton fluxes, RT-PCR, Stable membrane potential, Stable pH, Stable potassium concentration, Stable volume, Time Course Micro Array Experiment, Time courses of the internal pH changes, Volume determination during starvation at different times.
How does the Volume, internal pH, membrane potential and intracellular cation content change in a time dependent scale during potassium limitation.
Submitter: Falko Krause
Investigation: K+ Starvation in Saccharomyces cerevisiae
Assays: External Potassium Concentration, External pH changes, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, Membrane Potential, Protein Concentration, Time courses of the internal pH changes, Volume determination during starvation at different times.
