Volume determination during starvation at different times.
Is cell volume affected by potassium starvation?
The volume reduction as a response to the shift of cells from a medium of high potassium to potassium free medium will be studied in mutants lacking certain membrane transporters like Trk1,2 Nha1, etc. The conditions for the experiments follow Navarrete et al. (2010). Additionally knockouts of related regulation proteins (SAP155, SAP185) will be tested. For each mutant several time points will be measured to generate time courses.
SEEK ID: https://fairdomhub.org/assays/60
Experimental assay
Projects: TRANSLUCENT
Investigation: K+ Starvation in Saccharomyces cerevisiae
Study: Key Cell-Physiological Parameters
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 3rd Jun 2010 at 10:17
Last updated: 8th Nov 2017 at 14:21
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Projects: TRANSLUCENT
Institutions: Humboldt-Universität zu Berlin
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Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae
Programme: SysMO
Public web page: http://www.translucent-network.org/
Experimental approaches to evaluate cellular response to Potassium limitation. For this purpose, a series of isogenic strains derived from BY4741 and lacking one (TRK1 or TRK2) or both TRK genes encoding specific potassium uptake systems was constructed using the homologous recombination and Cre-lox system.
Submitter: Simon Borger
Studies: Analysis of proton and potassium fluxes, Experimental approaches to determine the dependence of volume, pH and me..., Ion Flux Changes, Key Cell-Physiological Parameters, Long Term Metabolomic Profile, Proteomic Studies, Pysiological Model, Transcriptional Profile
Assays: 2D-Gel Electrophoresis, Ammonium fluxes, External Potassium Concentration, External pH changes, HPLC-MS, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, LacZ Reporters, Membrane Potential, NMR, Potassium fluxes, Protein Concentration, Protein Identification - MS, Proton fluxes, RT-PCR, Stable membrane potential, Stable pH, Stable potassium concentration, Stable volume, Time Course Micro Array Experiment, Time courses of the internal pH changes, Volume determination during starvation at different times.
How does the Volume, internal pH, membrane potential and intracellular cation content change in a time dependent scale during potassium limitation.
Submitter: Falko Krause
Investigation: K+ Starvation in Saccharomyces cerevisiae
Assays: External Potassium Concentration, External pH changes, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, Membrane Potential, Protein Concentration, Time courses of the internal pH changes, Volume determination during starvation at different times.
