Investigations
What is an Investigation?
Investigation of how the ENA1 gene is transcriptionally regulated.
Experimental approaches to evaluate cellular response to Potassium limitation. For this purpose, a series of isogenic strains derived from BY4741 and lacking one (TRK1 or TRK2) or both TRK genes encoding specific potassium uptake systems was constructed using the homologous recombination and Cre-lox system.
Submitter: Simon Borger
Studies: Analysis of proton and potassium fluxes, Experimental approaches to determine the dependence of volume, pH and me..., Ion Flux Changes, Key Cell-Physiological Parameters, Long Term Metabolomic Profile, Proteomic Studies, Pysiological Model, Transcriptional Profile
Assays: 2D-Gel Electrophoresis, Ammonium fluxes, External Potassium Concentration, External pH changes, HPLC-MS, How does internal potassium change (/decrease) during several hours of p..., How starvation affect protein content in yeast cells, LacZ Reporters, Membrane Potential, NMR, Potassium fluxes, Protein Concentration, Protein Identification - MS, Proton fluxes, RT-PCR, Stable membrane potential, Stable pH, Stable potassium concentration, Stable volume, Time Course Micro Array Experiment, Time courses of the internal pH changes, Volume determination during starvation at different times.
Cellular parameters of strains lacking both TRK1 and TRK2 genes.
Submitter: Falko Krause
Studies: Bioinformatic studies, Current-voltage relation, Genetic analysis, Key Cell-Physiological Parameters of TRK1,2 Transport Systems Investiga..., Kinetic properties of Trk, Promotor Anaysis, Proteomic analysis, Transcriptional Profile, Transcriptomic Profiling of 14-3-3 Mutants
Assays: 2D Gel Analysis, Current voltage relation for different external KCl, Current-voltage relation for different internal potassium, Describing of membrane potential changes in response to proton fluxes., Determination of kinetic properties based on MIFE flux data and measurem..., In silico Promotor Anaysis, Insilico Promotor Anaysis, Interaction Database Analysis, Internal pH, Membrane Potential, Modelling of the Trk-current, Potassium Concentration, Protein Concentration, Volume
Investigation of the role of 14-3-3 proteins in the S. cerevisiae cation homeostasis
Submitter: Paul Heusden
Studies: Role of 14-3-3 proteins in the localization of proteins involved in cati...
Assays: No Assays
Experimental approaches to study the mechanism and ions involved in potassium uptake after long term potassium starvation.
Properties of cells lacking the NHA1 gene.
