Assays

Created At
Go
28 Assays visible to you, out of a total of 29

Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.

Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically

BPG degradation at 70C

No description specified
No description specified
No description specified

Submitter: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Enzymatic Activity Measurements

Investigation: 1 hidden item

Study: 1 hidden item

Kinetic characterisation of fructose 1,6-bisphosphate aldolase phosphatase

Temperature degradation of BPG, GAP and DHAP

Experimental data for the conversion of 3PG to F6P and the gluconeogenic pathway intermediates

The pilot experiment was conducted in order to create SOPs and to gain insight in transcriptome of cells grown at 70 and 80C

Samples obtained form the central fermentation facility of Sulfosys have been compared using iTRAQ (isobaric tag for relative and absolute quantification). A pilot experiment resulted in creation of SOP and initial data on cells grown at 70 and 80C

In order to get uniform data from all geographically separated partners in a consortium, central fermentation facility has been set-up. Based on empirical data, standard procedures for growing S. solfataricus cells have been developed.

Based on initial cell material, series of SOPs connected with assays of enzymes involved in Central Carbon Metabolism of S. solfataricus have been developed.

Pilot experiment concerning metabolome of S. solfataricus was conducted in order to acquire SOPs regarding the technique and gain insight on differences in metabolite concentrations at 70 and 80C

A model for the PGK reaction of yeast in presence or absence of the ATP recycling reactions

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-30C

BPG stability analysis

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: BPG stability

PGK 70C model

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-70C

PGK - GAPDH models

Submitter: Jacky Snoep

Biological problem addressed: Model Analysis Type

Investigation: Phosphoglycerate kinase acts as a futile cycle ...

Study: PGK-GAPDH 30C & 70C

Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

In vitro reconstitution of the PGK, GAPHD, TPI and FBPAase enzymes from S. solfataricus

Model prediction of the conversion of 3PG to fructose-6-phosphate and the gluconeogenic pathway intermediates. https://jjj.bio.vu.nl/models/experiments/kouril3_experiment-user/simulate

Mathematical model for GAPDH kinetics, saturation with BPG, NADPH, NADP, GAP and Pi

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Mathematical model for TPI kinetics, saturation with GAP and DHAP, and inhibition by 3PG and PEP

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Mathematical model for FBPAase kinetics, saturation with DHAP and GAP

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Modelling the degradation of BPG, GAP and DHAP at high temperature

Powered by
(v.1.12.0)
Copyright © 2008 - 2022 The University of Manchester and HITS gGmbH

By continuing to use this site you agree to the use of cookies