Assays

What is an Assay?
349 Assays visible to you, out of a total of 372

cca1 lhy Long #1 CCR2 marker

Property Value
BioDare ID 3648
Author Victoria Hibberd
Institution University of Edinburgh
License CC_BY

Description

If periods change during development, then our genetic screens for preiod-altering clock mutants might recover developmental mutants. Leaf movement assays suggestted that period might change. Ten-day data collections in the TopCount were intended to test for such period changes ...

cca1 lhy Long #1 CAB2 marker

Property Value
BioDare ID 3647
Author Victoria Hibberd
Institution University of Warwick University of Edinburgh
License CC_BY

Description

If periods change during development, then our genetic screens for preiod-altering clock mutants might recover developmental mutants. Leaf movement assays suggestted that period might change. Ten-day data collections in the TopCount ...

cca1 lhy Long #1 CCA1 marker

Property Value
BioDare ID 3638
Author Victoria Hibberd
Institution University of Edinburgh
License CC_BY

Description

If periods change during development, then our genetic screens for preiod-altering clock mutants might recover developmental mutants. Leaf movement assays suggestted that period might change. Ten-day data collections in the TopCount were intended to test for such period changes ...

AT0049

Property Value
BioDare ID 13558463723152
Author Sarah Hodge
Institution University of Edinburgh
License CC_BY

Description

LUC imaging10:10 blue+red into constant light

Purpose

testing acute light responses at dawn and dusk

Comments

27C BL-cryptochrome paper-rep3

Property Value
BioDare ID 13390776176796
Author Peter Gould
Institution University of Liverpool
License CC_BY

Description

27C, BL, on sucrose

Purpose

Temperature-comp-photoreceptor mutants

Comments

27C BL-cryptochrome paper-rep2

Property Value
BioDare ID 13390726557783
Author Peter Gould
Institution University of Liverpool
License CC_BY

Description

27C, BL, on sucrose

Purpose

Temperature-comp-photoreceptor mutants

Comments

27C BL-cryptochrome paper-rep1

Property Value
BioDare ID 13390707022980
Author Peter Gould
Institution University of Liverpool
License CC_BY

Description

27C, BL, on sucrose

Purpose

Temperature-comp-photoreceptor mutants

Comments

LUC CAB CCR2 MUT BL30 2LD to 5LL 17C 05/10/11

Property Value
BioDare ID 13185871416482
Author Peter Gould
Institution
License CC_BY

Description

BL 17C 2LD to 5LL

Purpose

To determine the effect of mutants on circadian clock in a temperature specific manner. RoBuST mutant library.

Comments

Part of a set of data from all RoBuST mutants looking at their effects on clock over a range of environmental conditions from RL, BL, RBL ...

AT0053: 4x3:21-LL

Property Value
BioDare ID 13790860334747
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging under 3:21 cycles then constant light. Replicate of experiment AT0064; similar to AT0007, AT0028.

Purpose

photoperiod alteration study

Comments

Imaging started 23/01/07. Fungal infection on GI and CCR2 cca1 lhy mutant plate. CAB, GI, CCA1, CCR2 cca1 lhy groups ...

AT0011 4x 12:12-LL

Property Value
BioDare ID 12730738745576
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

4 days of 12hrs light, 12hrs dark into 3 days constant light

Purpose

changes to photoperiod

Comments

AT0057: 4x12:12-LL

Property Value
BioDare ID 12730731549609
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 12:12 LD to LL in RB light

Purpose

testing different photoperiod effect on entrainment and phase on release to LL

Comments

CCR2:LUC in cca1 lhy and in cca1lhygi had white fungal contamination all over plate ELF3:LUC had low germination

AT0091: 4x12:12-LL

Property Value
BioDare ID 12730563219125
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 12L:12D into constant light

Purpose

testing variation of rhythmicity across camera field of view, in single seedlings with CCA1 or TOC1 marker

Comments

Original analysis by John O'Neill; Data curated by Andrew Millar.

Analysis of Control Strains Neurospora crassa

Property Value
BioDare ID 13945617808900
Author Felipe Munoz
Institution Pontificia Universidad Católica de Chile
License CC_BY

Description

Purpose

Compare different siblings of the parental/control strains in the global screening of transcription factors capable to affect the circadian oscilator

Comments

Entrainment Control Analysis

Property Value
BioDare ID 1178
Author Felipe Munoz
Institution Pontificia Universidad Católica de Chile
License CC_BY

Description

Purpose

Compare Period Calculations using entrainment and non entrainment strains for 72 hours

Comments

010514H1

Property Value
BioDare ID 1244
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

010514H2

Property Value
BioDare ID 1245
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

240414H1

Property Value
BioDare ID 1130
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

240414H2

Property Value
BioDare ID 1132
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

140515H1

Property Value
BioDare ID 3187
Author Sarah Hodge
Institution MPIMP - UEDIN
License

Description

At ZT0 dawn is dim light (6umolm-2s-1) for 3 hours. At ZT3 light steps up to 90 umolm-2s-1. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light (first day with double dawn) for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment based on Virginie and Thiago's design (Stitt Lab).

...

140515H2

Property Value
BioDare ID 3188
Author Sarah Hodge
Institution MPIMP - UEDIN
License

Description

ZT0 lights remain switched off until ZT3. At ZT3 light comes on at 90 umolm-2s-1. Dusk is lights off at ZT 12. Dark dawn cycle is for 4 days then continuous light (first day with dark dawn) for 3 days. ie 9L:15D x4 LLx3.

Purpose

Control "dark" dawn experiment for double dawn experiment based on Virginie and Thiago's design ...

280814H1

Property Value
BioDare ID 1927
Author Yvette Wilson
Institution
License

Description

At ZT0 dawn is dim light (1umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 4 days. ie 12L:12D x4 LLx4.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

280814H2

Property Value
BioDare ID 1928
Author Yvette Wilson
Institution
License

Description

6D: 6L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 280814H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

140814H1

Property Value
BioDare ID 1923
Author Yvette Wilson
Institution
License

Description

At ZT0 dawn is dim light (1umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

140814H2

Property Value
BioDare ID 1924
Author Yvette Wilson
Institution
License

Description

6D: 6L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 140814H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

one plate had very bad fungal contamination (plate 2). The samples affected are indicated in the 'experimental conditions'.

070814H1

Property Value
BioDare ID 1687
Author Yvette Wilson
Institution
License

Description

At ZT0 dawn is dim light (1 umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

070814H2

Property Value
BioDare ID 1688
Author Yvette Wilson
Institution
License

Description

12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 070814H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

030714H1

Property Value
BioDare ID 1646
Author Yvette Wilson
Institution
License

Description

At ZT0 dawn is dim light (6umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

030714H2

Property Value
BioDare ID 1647
Author Yvette Wilson
Institution
License

Description

12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 030714H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

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