ExploreData files for the conversion of XYL to KG, via the Caulobacter crescentus Weimberg pathway enzymes, omitting XLA: XDH, XAD, KDXD, KGSADH.
SEEK ID: https://fairdomhub.org/data_files/2859?version=2
Filename:
OnePotCascade-12.xlsx
Format: Spreadsheet
Size: 51.2 KB
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Created: 10th Apr 2019 at 14:11
Last updated: 6th Jan 2020 at 16:17
Last used: 25th Jan 2021 at 16:46
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Projects: Computational pathway design for biotechnological applications, ScyCode
Institutions: University Duisburg-Essen
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch, University of Manchester - Department of Computer Science, Stellenbosch University
The goal of the project is to establish a new biotechnological platform for the production of hydroxy-amino acids, since the current production of these important building blocks is very expensive. Enzyme engineering, systems biotechnology and metabolic engineering will be used in a synthetic biology approach.
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
The oxidative Weimberg pathway for the five-step pentose degradation to α ketoglutarate from Caulobacter crescentus is a key route for sustainable bioconversion of lignocellulosic biomass to added-value products and biofuels. Here, we developed a novel iterative approach involving initial rate kinetics, progress curves, and enzyme cascades, with high resolution NMR analysis of intermediate dynamics, and multiple cycles of kinetic modelling analyses to construct and validate a quantitative model
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Snapshots: Snapshot 1, Snapshot 2
One pot cascade - pathway analysis for the purified Caulinobacter crescentus Weimberg pathway enzymes. Effect of co-factor recycling, removal of XLA, and optimisation on Xylose to aKG is studied.
https://jjj.bio.vu.nl/models/experiments/shen2020_fig3a/simulate
https://jjj.bio.vu.nl/models/experiments/shen2020_fig3b/simulate
https://jjj.bio.vu.nl/models/experiments/shen2020_fig3c/simulate
https://jjj.bio.vu.nl/models/experiments/shen2020_fig3d/simulate
Person responsible: Jacky Snoep
Snapshots: No snapshots
Conversion of XYL to KG in one pot cascade of Weimberg pathway enzymes of Caulobacter crescentus, omitting XLA, measured in NMR.
https://jjj.bio.vu.nl/models/experiments/shen2020_fig3c/simulate
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Snapshots: Snapshot 1, Snapshot 2
