Tools: Fluorescence and confocal microscopy FRAP/FLIP, Molecular biology techniques (RNA/DNA), quantitative western blot analysis, fluorescence spectroscopy, rtPCR, Spectroscopy and structural analysis, Biochemistry and protein analysis
The focus of our research is the protein biogenesis and how stress-related factors modulate it. Protein biogenesis in general comprises various processes, i.e., translation, protein folding, each of which responds differently to external stress stimuli. Using systems biology approaches we seek to understand the interplay between these processes in fine-tuning the protein pattern and proteins’ abundance under osmotic stress conditions.
Tools: transcriptional analysis (Northern blots, Molecular biology techniques (RNA/DNA), quantitative western blot analyses, Proteomics (2D-PAGE), quantitative Western analyses), molecular biological techniques (RNA/DNA techniques, Fluorescence and confocal microscopy, PCR, Web services, Spectroscopy and structural analysis, Pharmacology and toxicology, Proteomics, Genetics
Postdoctoral Research fellow with experience in Genomics, transcriptomics, proteomics and metabolomics
Expertise: Systems Biology, regulation of gene expression, Signal transduction in Gram-negative bacteria; Synthetic Microbiology; Single cell gene expression; Regulatory networks; biochemistry; histidine ki..., stress responses, gene regulation, phenotypic heterogeneity, bistability, sporulation, translational control of gene expression, Bacillus subtilis, Molecular Biology, Genetics, Microbiology
Tools: Time-lapse fluorescence microscopy Flow cytometry, Molecular biology techniques (RNA/DNA), time lapse microscopy, Fluorecence based reporter gene analyses/single cell analyses, PCR, Single Cell analysis, Model organisms, Genetic analysis, Genetic modification, Molecular Biology, Genetics, Microbiology
Expertise: mRNA, overflow metabolism in Bacillus, carbon catabolite regulation in Gram positive bacteria, Clostridium, carbon metabolism, regulation of gene expression, Bacillus subtilis, Molecular Biology, Microbiology
Tools: microbiology techniques, Western blot analyses, reporter gene analyses, qRT-PCR, absolute quantification, Optimal experimental design, Molecular biology techniques (RNA/DNA), cultivation, northern blot analysis, Genetic modification