Studies

Following on in silico and in vitro work, the effect of MCAD deficiency on CoA metabolism was investigated. Using a recently published HILIC-MS/MS method, free and acylated CoA species could be measured simultaneously in HepG2 MCAD-KO cells. The levels of CoA biosynthesis intermediates and total CoA was also characterised by HPLC in liver samples from MCAD-KO mice exposed to energetic stress (fasting adn cold). qPCR was applied to investigate changes in the CoA metabolism that might constitute ...

Biomedical pre-trained language models (BioPLMs) have been achieving state-of-the-art results for various biomedical text mining tasks. However, prevailing fine-tuning approaches naively train BioPLMs on targeted datasets without considering the class distributions. This is problematic, especially with dealing with imbalanced biomedical gold-standard datasets for named entity recognition (NER). Regardless of the high-performing SOTA fine-tuned NER models, they are biased towards other (O) tags ...