SEEK ID: https://fairdomhub.org/people/1452
Location:
Netherlands
ORCID: Not specified
Joined: 11th Nov 2019
Expertise: Not specified
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PoLiMeR is funded through the EU Marie Skłodowska-Curie Innovative Training Network (ITN), which drives scientific excellence and innovation. ITNs bring together universities, research institutes, industry and clinical partners from across the world to train researchers to doctorate level.
Metabolic diseases are a burden on the European population and health care system. It is increasingly recognised that individual differences with respect to history, lifestyle, and genetic make-up affect disease ...
Programme: This Project is not associated with a Programme
Public web page: http://polimer-itn.eu/
Organisms: Homo sapiens, Mus musculus, Rattus norvegicus
Following on in silico and in vitro work, the effect of MCAD deficiency on CoA metabolism was investigated. Using a recently published HILIC-MS/MS method, free and acylated CoA species could be measured simultaneously in HepG2 MCAD-KO cells. The levels of CoA biosynthesis intermediates and total CoA was also characterised by HPLC in liver samples from MCAD-KO mice exposed to energetic stress (fasting adn cold). qPCR was applied to investigate changes in the CoA metabolism that might constitute ...
Submitter: Christoff Odendaal
Investigation: Mitochondrial fatty acid oxidation in human liver
Assays: Computational model (in silico), HepG2 cells (in vitro), Mice (in vivo)
Snapshots: Snapshot 1
Submitter: Christoff Odendaal
Biological problem addressed: Model Analysis Type
Investigation: Mitochondrial fatty acid oxidation in human liver
Organisms: No organisms
Models: Model notebooks, odendaal4
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
The behaviour of a published MCAD-KO HepG2 cell line was compared to that of a wild-type HepG2 cell line in terms of the changes in acyl-CoA and acylcarnitine levels (measured by HILIC-MS/MS) and gene expression pertaining to CoA metabolism (characterised by qPCR). Additionally, the incorporation of label from stable isotope-labelled pantothenate (vitamine B5) was over 24 hours of exposure to an energetic stressor was also investigated.
Submitter: Christoff Odendaal
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Mitochondrial fatty acid oxidation in human liver
Organisms: Homo sapiens (batch)
SOPs: No SOPs
Data files: HepG2 CoA labelling data, palmitate/no-glucose ..., HepG2 acyl-CoA measurements, palmitate/low-gluc..., HepG2 acyl-CoA measurements, palmitate/no-gluco..., HepG2 acyl-CoA/acylcarnitine measurements stati..., HepG2 acyl-CoA/acylcarnitine measurements stati..., HepG2 acylcarnitine measurements, palmitate/low..., HepG2 acylcarnitine measurements, palmitate/no-..., HepG2 qPCR statistical significance, palmitate/..., HepG2 qPCR statistical significance, palmitate/..., HepG2 qPCR, palmitate/low-glucose (Fig. S11), HepG2 qPCR, palmitate/no-glucose (Fig. S9)
Snapshots: No snapshots
MCAD-KO mice (C57BL/6J background) were exposed to three different conditions: "fed", "fasting", and "fasting + cold". In this study we determined the concentration of various CoA biosynthetic intermediates (including CoA) and the expression of CoA metabolic genes in liver tissue samples from these groups of mice.
Submitter: Christoff Odendaal
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Mitochondrial fatty acid oxidation in human liver
Organisms: No organisms
SOPs: No SOPs
Data files: Mice CoA biosynthetic intermediates (Fig. 4C), Mice qPCR (Fig. 4E-F)
Snapshots: No snapshots
Creators: Christoff Odendaal, Ligia Akemi Kiyuna, Madhulika Singh, Barbara Bakker, Amy HARMS, Thomas Hankemeier
Submitter: Christoff Odendaal
Model type: Not specified
Model format: Not specified
Environment: Not specified
Organism: Not specified
Investigations: Mitochondrial fatty acid oxidation in human liver
Studies: Sequestration of CoA and adaptation of CoA meta...
Assays: Computational model (in silico)
Abstract (Expand)
Authors: F. Bonanini, M. Singh, H. Yang, D. Kurek, A. C. Harms, A. Mardinoglu, T. Hankemeier
Date Published: 1st Apr 2024
Publication Type: Journal
PubMed ID: 38499143
Citation: Exp Cell Res. 2024 Apr 1;437(1):114008. doi: 10.1016/j.yexcr.2024.114008. Epub 2024 Mar 16.
Abstract (Expand)
Authors: Madhulika Singh, Ligia Akemi Kiyuna, Christoff Odendaal, Barbara M. Bakker, Amy C Harms, Thomas Hankemeier
Date Published: 11th Sep 2023
Publication Type: Journal
DOI: 10.26434/chemrxiv-2023-h0w52
Citation: [Preprint]
Abstract (Expand)
Authors: Z. Zhang, M. Singh, A. Kindt, A. B. Wegrzyn, M. J. Pearson, A. Ali, A. C. Harms, P. Baker, T. Hankemeier
Date Published: 31st Aug 2023
Publication Type: Journal
PubMed ID: 37696124
Citation: J Chromatogr A. 2023 Aug 31;1708:464342. doi: 10.1016/j.chroma.2023.464342.