Research Center Borstel

Mission: We perform cutting-edge research on data generated by novel biomolecular technologies to address questions in pneumology. Towards this, we use biological high-throughput data, i.e., OMICs data, to gain insights into molecular and phenotypic traits and diseases using computational methods. We combine data, methodology and their applications in pneumology through interdisciplinary collaboration with mathematicians, computer scientists, biologists and physicians.

This project collects summary information and (visual) characterizations of lung health related polygenic scores and underlying GWAS, based on publicly available data.

Background: The immunoproteasome is expressed constitutively in immune cells alongside standard proteasomes and forms multiple mixed types of proteasome complexes. These mixed 20S proteasomes may have distinct proteolytic activities depending on their subunit composition and may thereby regulate immune cell function. Using site-specific versus pan-inhibitors of the immunoproteasome, we here aim to dissect the biological functions of the three active sites of the immunoproteasome for monocyte cell ...

The conditions are Control: Control (no stimulation, no TLR8 inhibition) Inhib: TLR8 inhibition Msm: Methanobrevibacter smithii stimulation Mst: Methanosphaera stadtmanae stimulation Msm_Inhib: Methanobrevibacter smithii stimulation and TLR8 inhibition Mst_Inhib: Methanosphaera stadtmanae stimulation and TLR8 inhibition

Illumina RNA Sequencing of human PBMCs of 9 donors stimulated with two archaea, measured after 4 and 24 hours as well as without stimulation after 24 hours as control

A file combining all CpG sites from the differentially methylated regions (DMRs) of the control, t0, and t1 groups was generated. This combined file, containing methylation values extracted from the bedgraph files, was then used to create a PCA plot and a single heatmap for all CpG sites across the regions.

This compares the five conditions Control at 24 hours, Methanobrevibacter smithii (MBS) at 4 and 24 hours, Methanosphaera stadtmanae (MSS) at 4 and 24 hours in a joint model. The respective conditions are abbreviated Control_24h, MBS_4h, MBS_24h, MSS_4h, MSS_24h

This is a script to retrieve the beta-values for a specified genome region (hg38) from one Infinium Methylation array measurement. Input files for one sample are required to be in IDAT-format (RED + GREEN). For further information see README.txt