Investigations

What is an Investigation?
5 Investigations visible to you, out of a total of 18
Proteomic investigation of smooth muscle cells under PDGF/L1B vs.TGB1 stimulation

Human aortic smooth muscel cells (Thermo Fisher Scientific, C-007-5C, LOT: 2164581) were treated with TGB1 or PDGF/L1B to induce the contractile or synthetic SMC phenotype. As part of a multi-omics analysis, we conducted mass spectrometry to quantify protein levels of cell lysates using a HPLC-ESI-MS/MS system (HPLC=high pressure liquid chromatography, Ultimate 3000 UPLC system; ESI=electrospray ionization) and an Orbitrap Exploris™ 480 mass spectrometer. MS data were analyzed and normalized with ...

Submitter: Tobias Reinberger

Studies: Protein quantification using mass spectrometry

Assays: Differential protein level anaylsis (C3 vs. C4), Raw maxLFQ protein intensities (C3 vs. C4)

Illumina RNA Sequencing
No description specified

Submitter: Inken Wohlers

Studies: Differential Expression, Transcriptome profiling

Assays: Comparison C2-C1, Comparison C3-C1, Comparison C3-C2, Comparison C3-C4, Comparison C3-C5, Comparison of all conditions (C1,C2,C3,C4,C5), Expression profiling with heatmaps and PCA

ONT direct RNA Sequencing
No description specified

Submitter: Ayat Ismail

Studies: Differential transcript expression analysis, RNA modification detection

Assays: Comprehensive RNA Modification Analysis and Visualization, Differential gene expression and pathway enrichment using Deseq2

Illumina RNA sequencing of archaean stimulated human monocytes and under TLR8 inhibition

The conditions are Control: Control (no stimulation, no TLR8 inhibition) Inhib: TLR8 inhibition Msm: Methanobrevibacter smithii stimulation Mst: Methanosphaera stadtmanae stimulation Msm_Inhib: Methanobrevibacter smithii stimulation and TLR8 inhibition Mst_Inhib: Methanosphaera stadtmanae stimulation and TLR8 inhibition

Submitter: Inken Wohlers

Studies: Differential expression, Transcriptome profiling

Assays: Comparison of all conditions, Expression profiling with heatmaps and PCA

Illumina RNA sequencing of archaean stimulated human PBMCs

Illumina RNA Sequencing of human PBMCs of 9 donors stimulated with two archaea, measured after 4 and 24 hours as well as without stimulation after 24 hours as control

Submitter: Inken Wohlers

Studies: Differential expression, Transcriptome profiling

Assays: Comparison of all conditions, Expression profiling with heatmaps and PCA

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