Differential protein level anaylsis (C3 vs. C4)
Cell line: Human aortic smooth muscel cells (Thermo Fisher Scientific, C-007-5C, LOT: 2164581)
Technical replicates: n=4 per condition
Conditions: C3 = TGFB1-treated SMCs C4 = PDGF-BB/IL1B-treated SMCs
SEEK ID: https://fairdomhub.org/assays/2682
Modelling analysis
Projects: OMICS of vascular smooth muscle cells under oxidative stress
Investigation: Proteomic investigation of smooth muscle cells under PDGF/L1B vs.TGB1 stimulation
Study: Protein quantification using mass spectrometry
Assay position:
Biological problem addressed: Model Analysis Type
Organisms: No organisms
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Created: 18th Jun 2025 at 10:05
Last updated: 19th Dec 2025 at 13:08
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https://orcid.org/0000-0002-1507-0402
The FZB is the lung research center of the Leibniz Association and is therefore committed to the motto “theoria cum praxi”.
The FZB sees its scientific and socio-political task in basic, disease- and patient-oriented lung research and training in this field. The field of research includes chronic inflammatory diseases of the lung of infectious and non-infectious environmental and behavioral origin. Research focuses on tuberculosis, infections with non-tuberculous mycobacteria and other bacterial ...
Projects: Group Data Science, Group Microbial Interface Biology, Core Facility Fluorescence Cytometry, Targeting the immunoproteasome: Analyzing the biological effects of specific versus pan inhibition, Macrophage and neutrophil redox proteome in infection with Mycobacterium tuberculosis, Methylation differences during respiratory infection, Lipid droplet formation and dynamics in murine macrophages, OMICS of vascular smooth muscle cells under oxidative stress, Systems genetics of lung health and asthma (GWAS, PGS, QTL), Archaean stimulation of human primary PBMCs and PBMCs monocytes
Web page: https://fz-borstel.de
Programme: Research Center Borstel
Public web page: Not specified
Human aortic smooth muscel cells (Thermo Fisher Scientific, C-007-5C, LOT: 2164581) were treated with TGB1 or PDGF/L1B to induce the contractile or synthetic SMC phenotype. As part of a multi-omics analysis, we conducted mass spectrometry to quantify protein levels of cell lysates using a HPLC-ESI-MS/MS system (HPLC=high pressure liquid chromatography, Ultimate 3000 UPLC system; ESI=electrospray ionization) and an Orbitrap Exploris™ 480 mass spectrometer. MS data were analyzed and normalized with ...
Creators: Tobias Reinberger, Elke Hammer
Submitter: Tobias Reinberger
Relationship type: Not specified
