Assays

This compares the five conditions Control at 24 hours, Methanobrevibacter smithii (MBS) at 4 and 24 hours, Methanosphaera stadtmanae (MSS) at 4 and 24 hours in a joint model. The respective conditions are abbreviated Control_24h, MBS_4h, MBS_24h, MSS_4h, MSS_24h

A file combining all CpG sites from the differentially methylated regions (DMRs) of the control, t0, and t1 groups was generated. This combined file, containing methylation values extracted from the bedgraph files, was then used to create a PCA plot and a single heatmap for all CpG sites across the regions.