SOPs

This protocol is designed to prepare sufficient amounts of high-quality total RNA from the yeast saccharomyces cerevisiae grown in liquid culture for analysis on microarrays.

Creators: Walter Glaser, Christa Gregori

Submitter: Walter Glaser

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 26th Oct 2009 at 14:48, Last updated: 20th May 2010 at 08:45

Q/QH2 sampling

SUMO

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Steady state studies for different oxygen avail...

Studies: The Escherichia coli steady state response to o...

Assays: Determination of intracellular redox state by m...

Created: 16th Mar 2010 at 17:14

Measurement of extracellular metabolites

SUMO

No description specified

Creator: Sonja Steinsiek

Submitter: The JERM Harvester

Investigations: Analysis of Escherichia coli with linear electr..., Analysis of the glucose transport in Escherichi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Characterization of mutant strains with defects..., Determination of the impact of specific enzyme ...

Assays: Aerobic and anaerobic characterization of MG165..., Analysis of by-product formation rates in MG1655, Characterization of E. coli MG1655 and ∆sdhC an..., Determination of by-product formation and gluco...

Created: 16th Mar 2010 at 17:13

Evans Medium

SUMO

No description specified

Creator: Katja Bettenbrock

Submitter: The JERM Harvester

Investigations: Analysis of Escherichia coli with linear electr..., Dynamical studies for different oxygen availabi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Determination of the impact of specific enzyme ..., The Escherichia coli dynamical response to oxyg..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Analysis of gene expression rates at different ..., ArcA phosphorylation at different aerobiosis le..., Characterization of E. coli MG1655 and ∆sdhC an..., FNR activity at different aerobiosis levels (st..., Gene expression analysis of mutants with linear..., Physiological measurements from Sheffield chemo..., Transcriptional profiling of E. coli during ana...

Created: 16th Mar 2010 at 17:13

DCW measurement

SUMO

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Steady state studies for different oxygen avail...

Studies: Determination of the impact of specific enzyme ..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Determination of intracellular metabolite conce..., Determination of intracellular redox state by m..., FNR activity at different aerobiosis levels (st..., Physiological measurements from Sheffield chemo...

Created: 16th Mar 2010 at 17:12

PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Mem

TRANSLUCENT

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins

Creator: Miguel Curto Rubio

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 12th Mar 2010 at 16:57

PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Cit

TRANSLUCENT

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins

Creator: Miguel Curto Rubio

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 12th Mar 2010 at 16:57

Cation content analysis - uco

TRANSLUCENT

Purpose: Cation content analysis - protocol

Creator: Clara Navarrete

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 12th Mar 2010 at 16:57

Potassium starvation protocol

TRANSLUCENT

Purpose:

Creator: Jose Ramos

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 12th Mar 2010 at 16:57

ß-Galactosidase assay

BaCell-SysMO

SOP for ß-Galactosidase assay.

Creator: Praveen kumar Sappa

Submitter: Praveen kumar Sappa

Investigations: The transition from growing to non-growing Baci...

Studies: Controlled sigmaB induction in shake flask

Assays: IPTG induction of sigmaB in BSA115

Created: 17th Nov 2009 at 09:11, Last updated: 17th Nov 2009 at 09:14

SOP for cultivation of B.Subtilis

BaCell-SysMO

SOP for shake flask cultivation of B.Subtilis in Bacell-Sysmo

Creator: Praveen kumar Sappa

Submitter: Praveen kumar Sappa

Investigations: Redefining the Complete Transcriptome of Bacill..., The transition from growing to non-growing Baci...

Studies: Controlled sigmaB induction in shake flask, Transcriptome analysis of glucose starvation in...

Assays: IPTG induction of sigmaB in BSA115, Tiling Array analysis of glucose strarved B. su...

Created: 17th Nov 2009 at 09:14

SUMO mRNA isolation Epicentre

SUMO

This file describes how to isolate mRNA from E. coli using the kit from Epicentre, for gene expression analysis via RT-PCR

Creator: Sonja Steinsiek

Submitter: Sonja Steinsiek

Investigations: Analysis of Escherichia coli with linear electr..., Analysis of the glucose transport in Escherichi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Characterization of mutant strains with defects..., Determination of the impact of specific enzyme ...

Assays: Aerobic and anaerobic characterization of MG165..., Analysis of gene expression rates at different ..., Characterization of MG1655 and mutant strains u..., Gene expression analysis of mutants with linear...

Download

Created: 26th Oct 2009 at 14:48

SUMO chemostat conditions

SUMO

This document describes the chemostat conditions to run comparable experiments in different bioreactors in different labs.

Creator: Sonja Steinsiek

Submitter: Sonja Steinsiek

Investigations: Analysis of Escherichia coli with linear electr..., Analysis of the glucose transport in Escherichi..., Dynamical studies for different oxygen availabi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Characterization of mutant strains with defects..., Determination of the impact of specific enzyme ..., The Escherichia coli dynamical response to oxyg..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Analysis of gene expression rates at different ..., ArcA phosphorylation at different aerobiosis le..., Characterization of MG1655 and mutant strains u..., Determination of by-product formation and gluco..., Determination of intracellular metabolite conce..., Determination of intracellular redox state by m..., Gene expression analysis of mutants with linear..., Physiological measurements from Sheffield chemo..., Transcriptional profiling of E. coli during ana...

Download

Created: 26th Oct 2009 at 14:48

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