Muscle samples (salt water sampling) - Identification of compounds based on the LipidBlast database.
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Created: 12th Mar 2018 at 01:47
Last updated: 20th Aug 2021 at 10:22
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Version 1 (earliest) Created 12th Mar 2018 at 01:47 by Zdenka Bartosova
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Projects: GenoSysFat, DigiSal
Institutions: Norwegian University of Life Sciences
https://orcid.org/0000-0003-4882-2188Systems biology for salmon farming is what I do. I lead the DigiSal project (http://tinyurl.com/digisal), whose full title is "Towards the Digital Salmon: From a reactive to a pre-emptive research strategy in aquaculture". DigiSal is part of Digital Life, the first call dedicated to systems biology by the Research Council of Norway. I'm also one of the lead modellers in GenoSysFat (http://tinyurl.com/genosysfat), working to improve the omega-3 content of salmon farmed on sustainable feeds by ...
Salmon farming in the future must navigate conflicting and shifting demands of sustainability, shifting feed prices, disease, and product quality. The industry needs to develop a flexible, integrated basis of knowledge for rapid response to new challenges. The Digital Salmon will be an ensemble of mathematical descriptions of salmon physiology, combining mathematics, high-dimensional data analysis, computer science and measurement technology with genomics and experimental biology into a concerted ...
Projects: GenoSysFat, DigiSal, SEEK tutorial for DigiSal, DigiSal-BT8121
Web page: http://tinyurl.com/digisal
Towards the Digital Salmon: From a reactive to a pre-emptive research strategy in aquaculture (DigiSal)
Salmon farming in the future must navigate conflicting and shifting demands of sustainability, shifting feed prices, disease, and product quality. The industry needs to develop a flexible, integrated basis of knowledge for rapid response to new challenges. Project DigiSal will lay the foundations for a Digital Salmon: an ensemble of mathematical descriptions of salmon physiology, combining ...
Programme: The Digital Salmon
Public web page: http://tinyurl.com/digisal
Organisms: Danio rerio, Salmo salar, Oncorhynchus mykiss
Salmon farmed on modern feeds contains less of the healthy, long-chain fatty acids (EPA and DHA) than before. Up until the turn of the millennium, farmed salmon were fed fish oil as a replacement for their omega-3 rich natural prey. However, fish oil is now a scarce resource, and more than half of the fat in modern feeds comes from plant oils that are inexpensive, but devoid of long-chain omega-3 fatty acids. How can we increase the omega-3 content of salmon on sustainable feeds?
One option is ...
Programme: The Digital Salmon
Public web page: http://tinyurl.com/genosysfat
Organisms: Danio rerio, Salmo salar, Oncorhynchus mykiss
Atlantic salmon is a main source of essential ω-3 long-chain polyunsaturated fatty acids (LC-PUFA) in many Western diets, especially eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA). However, farmed salmon meat now contains less healthy ω-3 fatty acids (FA) than before, due to the shift from marine to vegetable lipid feed sources. An important future aquaculture challenge is therefore to maintain a healthy and high EPA/DHA content when farmed salmon is fed a vegetable ...
Submitter: Jon Olav Vik
Studies: GSF1: Salmon feed-switch experiment vegetable and fish oil 2015-2016
Assays: Fatty acid contents in feed using Gas chromatography/FAME analysis, Fatty acid contents in tissues using Gas chromatography/FAME analysis, Feed switch 2015-09 Solbergstranda, pilot proteomics, Feed switch 2015-09, 2016-01 Solbergstranda, gross phenotypes, Feed switch 2015-09, 2016-01 Solbergstranda, gut microbiota composition,..., Lipidomics, Metabolomics, Overview of RNAseq datasets in GenoSysFat, RNA sequencing Feed switch- Liver and Gut
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This experiment is designed to pinpoint where in the metabolic network there are differences between salmon of different genetic families and on different diets. Analyses of this material will help inform feeding and breeding strategies.
Salmon will be reared on feeds with contrasting levels of very-long-chain polyunsaturated fatty acids. Then some fish will be crossed over to the other diet while others remain as controls. This perturbation of diet should provoke changes in omega-3 metabolism ...
Submitter: Jon Olav Vik
Investigation: Omega-3 metabolism of salmon in relation to die...
Assays: Fatty acid contents in feed using Gas chromatography/FAME analysis, Fatty acid contents in tissues using Gas chromatography/FAME analysis, Feed switch 2015-09 Solbergstranda, pilot proteomics, Feed switch 2015-09, 2016-01 Solbergstranda, gross phenotypes, Feed switch 2015-09, 2016-01 Solbergstranda, gut microbiota composition,..., Lipidomics, Metabolomics, Overview of RNAseq datasets in GenoSysFat, RNA sequencing Feed switch- Liver and Gut
Snapshots: Snapshot 1
NB! The files here are the old version for the files in Lipidomics (Experimental Assay)
Lipidomic analysis by LC-MS of tissue samples from the GSF1 feed-switch experiment. Samples were analyzed at NTNU by Zdenka Bartosova and Per Bruheim.
There are two separate data files of lipid analysis in muscle and liver samples. Excel sheets contains both raw and normalised data of compounds abundance. Normalization to all compounds was used as a normalization method.
We have also performed a "normalization ...
Submitter: Jon Olav Vik
Assay type: Experimental Assay Type
Technology type: Liquid Chromatography Mass Spectrometry
Investigation: Omega-3 metabolism of salmon in relation to die...
Organisms: Salmo salar
SOPs: SOP_Lipid analysis
Data files: NEG FW_Liver_Tentative Compounds Identification, NEG FW_Muscle_Tentative Compounds Identification, NEG Fresh Water Liver, NEG Fresh Water Muscle, NEG SW_Liver_Tentative Compounds Identification, NEG SW_Muscle_Tentative Compounds Identification, NEG_Salt Water Liver, NEG_Salt Water Muscle, POS FW_Liver_Tentative Compounds Identification, POS FW_Muscle_Tentative Compounds Identification, POS_ Fresh Water Liver, POS_ Fresh water Muscle, POS_Normalization_experiment, POS_Salt Water Liver, POS_Salt Water Muscle, Preliminary principal component analysis, SW_Liver_Tentative Compounds Identification- Po..., SW_Muscle_Tentative Compounds Identification- P...
Snapshots: No snapshots