SDtoLD 260410
SDtoLD 260410
| Property | Value |
|---|---|
| BioDare ID | 13982031569136 |
| Author | Laura Dixon |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
pTOC plate 0683 pCAB plate0684 LHY plate 0685 control plate 0686 TOC plate0687 pLHY plate 0688
Purpose
Re-entrainment dynamics during the transition between light:dark cycles with different photoperiods, comparing Arabidopsis thaliana and Ostreococcus tauri. This expt in O. tauri compares transcriptional LUC fusions to TOC1, CAB, and LHY promoters with translational fusions to LHY and TOC promoter + protein. Each marker is on a separate plate to avoid plate-position effects.
Comments
Several controls are relevant, from parallel experiments in adjacent weeks. Data displayed in BioDare are means of 8 cultures in a 96-well plate column, where data from each culture was normalised to its mean before averaging. 6 such columns were measured for each marker (each marker has its own plate). Raw data are shown in the 'normalised' files. Aggregated data in the 'summary.xls' file. NB each plate is counted in sequence, so necessarily at a different time. The four LHY/TOC1 markers are counted within 0.5h, in a total cycle time of 0.707h. For display in BioDare, the times are shown for the middle plate, TOC, but the separate files have the original, separate times. The peak analysis in the publication used the averages for each column.
SEEK ID: https://fairdomhub.org/assays/2922
Experimental assay
Projects: BioDare1
Investigation: Dixon Laura
Study: Dixon et al. (2014)
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: Ostreococcus tauri
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Created: 18th Jun 2026 at 12:55
Last updated: 18th Jun 2026 at 12:59
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https://orcid.org/0000-0002-1352-7245Research Software Engineer in the Biological Research Data Management team (BioRDM) at the University of Edinburgh.
The Centre for Engineering Biology is the University of Edinburgh's research organisation in interdisciplinary, Synthetic and Systems Biology, founded in 2023 as the successor to SynthSys, itself founded in 2012 as the successor to the Centre for Systems Biology at Edinburgh (CSBE). CSBE was newly founded in 2007. The Centre for Engineering Biology is a hub organisation that links across multiple, major funding awards, including an Engineering Biology Mission Hub, and EPSRC C-loop.
Projects: Millar group, PHYTOCAL: Phytochrome Control of Resource Allocation and Growth in Arabidopsis and in Brassicaceae crops, TiMet, POP - the Parameter Optimisation Problem, Regulation of flowering time in natural conditions, PlaSMo model repository, BioDare1
Web page: https://biology.ed.ac.uk/centre-engineering-biology
Experiment data previously hosted on the original version of BioDare (biodare.ed.ac.uk).
Programme: Centre for Engineering Biology (prev. SynthSys)
Public web page: Not specified
Collection of experiments created by Dixon Laura on the original BioDare and automatically transferred to FAIRDOM Hub.
Light and circadian regulation of clock components aids flexible responses to environmental signals
Dixon et al. (2014)
DOI: 10.1111/nph.12853
summary (from BioDare)
TOC normalised (from BioDare)
pTOC 0683 normalised (from BioDare)
pLHY normalised (from BioDare)
pCAB plate0684Derandomized (from BioDare)
LHY 0685 normalised (from BioDare)
control plate 0686Derandomized (from BioDare)
STANDARD_DATAFILE.2 (from BioDare)
STANDARD_RAWDATAFILE.2 (from BioDare)
Ot data summary for BioDare 2014 v3.1 (from BioDare)
List of samples used in the assay (extracted from original BioDare metadata and converted to csv)
Original BioDare metadata, converted to json format
Readme file
