Projects: Simulation Foundries, CML for thermophysical properties of mixtures, Test Project (dummy), Towards Reproducible Enzyme Modeling
Institutions: University of Stuttgart, Karlsruhe Institute of Technology (KIT)
https://orcid.org/0000-0001-9119-1778Expertise: enzyme kinetics, enzymes, Enzymatic reactions, biochemical enzyme characterization, Biochemistry, molecular simulation, molecular modeling, Programming, Bioinformatics, Computational Biology
Tools: Gromacs, Python, Molecular Dynamics, bash, Biochemistry, Bioinformatics, Biochemistry and protein analysis, Enzyme assay, enzyme kinetics, isothermal titration calorimetry, dynamic light scattering, Spectrophotometry
Polyglot European Scientist. I thrive working in interdisciplinary environments combining the study of enzyme reactions and mechanisms with bioinformatics, molecular modelling, automated data analysis and data stewardship.
Raw data of the measurements obtained via DLS measurements. Can be opened with the Zetasizer software.
Raw data of the measurements obtained via DLS measurements. Can be opened with the Zetasizer software.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...
Studies: DLS measurements (homogeneity of an enzyme sample)
Assays: DLS measurements in 2 buffers
Raw data of the measurements obtained via DLS measurements. Can be opened with the Zetasizer software.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Gre2p activity monitored by NADPH absorbance using different enzyme concentrations. Activity is measured after different storage conditions (treatments) and in presence of different amounts of tween. Also analysis of DLS data of homogeneity of Gre2p samples measured before and after these treatments.
A python workflow is used to analyse the data and create a plot of the data. It requires the following directory structure:
./Script_for_S13andS18.py ./Script_for_S22.py ./Script_for_S15.py
and as ...
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...
Studies: DLS measurements (homogeneity of an enzyme sample), Spectrophotometric Activity Measurements
Assays: DLS measurements in 2 buffers, DLS measurements in KPi Buffer and in KPi buffe..., DLS measurements in KPi buffer with BSA added, Specific activity of Gre2p
SOP describing how to perform Dynamic Light Scattering (DLS) measurements with Gre2p in cuvettes using a Zetasizer Nano DLS reader.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli