Miniaturized and automated adaptive laboratory evolution: Evolving Corynebacterium glutamicum towards an improved d-xylose utilization.

Abstract:

Adaptive Laboratory Evolution (ALE) is increasingly being used as a technique for untargeted strain optimization. This work aimed at developing an automated and miniaturized ALE approach based on repetitive batch cultivations in microtiter plates. The new method is applied to the recently published strain Corynebacterium glutamicum pEKEx3-xylXABCDCc, which is capable of utilizing d-xylose via the Weimberg (WMB) pathway. As a result, the significantly improved strain WMB2evo was obtained, showing a specific growth rate of 0.26h-1 on d-xylose as sole carbon and energy source. WMB2evo grows stable during lab-scale bioreactor operation, demonstrating the high potential of this strain for future biorefinery applications. Genome sequencing of cell samples from two different ALE processes revealed potential key mutations, e.g. in the gene cg0196 (encoding for the transcriptional regulator IolR of the myo-inositol metabolism). These findings open up new perspectives for the rational engineering of C. glutamicum towards improved d-xylose utilization.

FAIRDOMHub ID: https://fairdomhub.org/publications/344

PubMed ID: 28552568

Projects: XyloCut

Journal: Bioresour Technol

Citation: Bioresour Technol. 2017 May 12. pii: S0960-8524(17)30709-5. doi: 10.1016/j.biortech.2017.05.055.

Date Published: 30th May 2017

Authors: A. Radek, Niklas Tenhaef, M. F. Muller, Christian Brüsseler, W. Wiechert, Jan Marienhagen, T. Polen, Stephan Noack

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Created: 29th Aug 2017 at 12:08

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