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Christian Brüsseler

About Christian Brüsseler:
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SEEK ID: https://fairdomhub.org/people/863

Location:  Germany

Expertise: Not specified

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ORCID: https://orcid.org/0000-0003-0128-7827

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XyloCut (Forschungszentrum Jülich)

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XyloCut

D-xylose is a major component of lignocellulose and is after D-glucose the most abundant monosaccharide on earth. However, D-xylose cannot be naturally utilised by several industrially relevant microorganisms. On the way to a strong bio-based economy in Europe, this widely available feedstock has to be made accessible for the sustainable microbial synthesis of value-added chemical building blocks to be used in a broad range of applications. The project aims at engineering Corynebacterium glutamicum
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Programme: ERASysAPP

Public web page: https://www.erasysapp.eu/calls/2nd-call/xylocut

Miniaturized and automated adaptive laboratory evolution: Evolving Corynebacterium glutamicum towards an improved d-xylose utilization.
XyloCut

Abstract (Expand)

Adaptive Laboratory Evolution (ALE) is increasingly being used as a technique for untargeted strain optimization. This work aimed at developing an automated and miniaturized ALE approach based on … repetitive batch cultivations in microtiter plates. The new method is applied to the recently published strain Corynebacterium glutamicum pEKEx3-xylXABCDCc, which is capable of utilizing d-xylose via the Weimberg (WMB) pathway. As a result, the significantly improved strain WMB2evo was obtained, showing a specific growth rate of 0.26h-1 on d-xylose as sole carbon and energy source. WMB2evo grows stable during lab-scale bioreactor operation, demonstrating the high potential of this strain for future biorefinery applications. Genome sequencing of cell samples from two different ALE processes revealed potential key mutations, e.g. in the gene cg0196 (encoding for the transcriptional regulator IolR of the myo-inositol metabolism). These findings open up new perspectives for the rational engineering of C. glutamicum towards improved d-xylose utilization.

Authors: A. Radek, Niklas Tenhaef, M. F. Muller, Christian Brüsseler, W. Wiechert, Jan Marienhagen, T. Polen, Stephan Noack

Date Published: 30th May 2017

Journal: Bioresour Technol

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