Assays
What is an Assay?Filters
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
This assay captures metadata associated with bacterial extraction procedures, documenting the experimental parameters, sample identifiers, reagents, and procedural conditions used during the isolation and extraction of bacterial material from biological or environmental samples. It serves as a standardized record-keeping component that accompanies downstream analytical assays, ensuring traceability and reproducibility by cataloging key variables such as sample source, extraction method, instrument ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
DNA extraction is a fundamental laboratory procedure used to isolate and purify genomic DNA from biological samples for downstream molecular analyses. This assay encompasses the collection of relevant metadata associated with the extraction process, including sample identifiers, source material type, extraction method or kit utilized, instrument parameters, operator information, and quality control metrics such as DNA yield and purity (e.g., A260/A280 ratios). Standardized metadata capture ensures ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay quantifies gene expression at single-cell resolution by linking individual cell transcriptomic profiles to associated experimental or clinical data, enabling integrative analysis across multiple data modalities. Single-cell RNA sequencing (scRNA-seq) technology is employed to capture and measure the transcriptional activity of individual cells, generating high-dimensional expression matrices that are subsequently processed through quality control, normalization, and dimensionality ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Positron emission tomography combined with computed tomography (PET-CT) is a hybrid nuclear medicine imaging assay that simultaneously measures functional metabolic activity and anatomical structure within the body. Radioactive tracers (typically 18F-fluorodeoxyglucose, FDG) are administered to the subject, and their differential uptake is detected by the PET component to quantify metabolic or biochemical processes, while the CT component provides high-resolution structural localization. The "Data ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Immunohistochemistry (IHC) is a histological technique that detects and localizes specific proteins or antigens within tissue sections through the use of labeled antibodies, enabling assessment of protein expression, distribution, and abundance in a spatially resolved context. Tissue samples are typically fixed, embedded, sectioned, and subjected to antigen retrieval prior to incubation with primary and secondary antibodies conjugated to detectable reporters such as enzymatic or fluorescent labels. ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures administrative and clinical metadata associated with patient study visits, serving as a structured data collection framework rather than a biological measurement assay. It records visit-level contextual information such as visit dates, timepoints, subject identifiers, clinical site details, and protocol-relevant annotations that are essential for linking experimental samples and assay results to their proper clinical context. This metadata layer ensures traceability and data ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures metadata associated with tissue collection procedures, documenting critical provenance and contextual information for biological specimens obtained from study subjects. It records standardized parameters such as tissue type, anatomical source, collection method, preservation conditions, and relevant donor or sample identifiers to ensure traceability and reproducibility across downstream experimental workflows. This metadata serves as a foundational record linking collected ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB