Assays

Liver samples from Atlantic cod males. Total RNA isolated using TRI Reagent (Fekadu Yadetie). Sequencing performed with Illumina Stranded mRNA (sequencing poly-A mRNA) at Genomic Core Facility at UiB/Haukeland (contact person Rita Holdhus).

The data sets are submitted to the ArrayExpress repository, and will be linked here.

Untargeted lipidomics analysis was performed on plasma and liver samples of four male fish from each group (n = 4) at Per Bruheim’s lab at NTNU, Norway, using Liquid Chromatography Hybrid Quadrupole Mass Spectrometry (UPLC-HDMS).

The data is submitted to the PRIDE repository, and will be linked here.

Targeted lipidomic analysis was performed on plasma and isolated liver microsomes of eight male fish from solvent control (Control) and High-Dose groups (n = 8) at Cinta Porte’s lab at CSIC, Spain, using Flow Injection Analysis High-Resolution Mass Spectrometry (FIA-HRMS).

The data is submitted to the MetaboLights repository.

Fish weight (start - end), length, total growth and specific growth rate (SGR)

Build the chemical defensome gene list for 5 fish: Zebrafish (Danio rerio), Atlantic cod (Gadus morhua), medaka (Oryzias latipes), Atlantic killifish (Fundulus heteroclitus) and stickleback (Gasterosteus aculeatus). Source code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/defensomeGenes

To study the defensome genes' expression in early developmental stages of zebrafish and stickleback. Souce code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/developmentalStages

Exposing zebrafish to benzo(a)pyrene (B(a)P) (gene counts from NCBI GEO: GSE64198, previously published by Fang, et al. 2015. Souce code and relevant files can be found on GitHub: https://github.com/zhxiaokang/fishDefensome/tree/main/exposureResponse